Molecular Imaging: Principles And Applications In Biomedical Research

Product Description

Molecular imaging is a rapidly emerging field that translates many concepts developed for molecular biology and cellular imaging to the in vivo imaging of intact organisms. The technique allows the study of molecular biological events in their full context and will therefore become an indispensable tool for biomedical research and drug discovery and development. This volume familiarizes the reader with the concepts of imaging and molecular imaging in particular. Basic principles of imaging technologies, reporter moieties for the various imaging modalities and the design of target reporter constructs are described in the first part. The second part illustrates how these tools can be used to visualize relevant molecular events: the biodistribution of drugs/ligands, the expression of drug targets (receptors, enzymes), and the consequences of the molecular drug-target interactions (pathway activations, system responses). A final chapter deals with visualization of cell migration (cell therapies).

Customer Reviews:

Summarizes the Current State of the Art.......2006-03-11

It seems that with the ability to get any signal at all through a body, the modern day imaging systems can produce an image with useful information. In this book various signal generating systems (I guess you would call them) be it X-Ray tomography, magnetic resonance, and nuclear imaging are discussed.

This is followed with a discussion on the various techniques for reporting the images. Finally the book discusses the uses of these imaging techniques in examining the actions of drugs in living animals. These techniques are non-invasive so that what the drug is doing can be observed in real time. This is extremely important as getting the drug to the proper location in the patient where it can work is of the utmost in importance. Other uses of the technology are likewise discussed such as cell migration.

This book represents a good summary of the state of the art as it exists today and points in some of the directions that research is going in this rapidly emerging field.

Fluorescent Energy Transfer Nucleic Acid Probes: Designs And Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)

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Fluorescent Energy Transfer Nucleic Acid Probes: Designs And Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588293807

Book Description

The first comprehensive treatment of energy transfer (ET) nucleic acid probes. Hands-on experts thoroughly describe all the major probes, both fluorescence resonance energy transfer (FRET)-based and non-FRET-based, and provide a complete set of techniques to monitor DNA and RNA reactions, including hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Optimal design strategies for customized ET probes are presented, as well as techniques for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, DNA breaks and single nucleotide polymorphisms. Merging work on nanotechnology, and fluorescent probes, authors describe in detail the design and application of ET-using molecular devices, such as biosensors, molecular machines, and logic gates for molecular scale computation.

Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology)

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology)
Peter G. Isaac
Manufacturer: Humana Press
ProductGroup: Book
Binding: Paperback

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ASIN: 089603254X

Book Description

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

Diagnostic Molecular Microbiology: Principles and Applications

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Diagnostic Molecular Microbiology: Principles and Applications
David H. Persing , Thomas F. Smith , Fred C. Tenover , and Thomas J. White
Manufacturer: American Society Microbiology
ProductGroup: Book
Binding: Plastic Comb

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ASIN: 155581056X

The Rock from Mars: A Detective Story on Two Planets

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Rock from Mars Review
Human Reaction In the Face of a Possible Paradigm Shift
Tales of the Rock Star
Science vs Politics. (Guess who wins?)
A Story of Big Science

The Rock from Mars: A Detective Story on Two Planets
Kathy Sawyer
Manufacturer: Random House
ProductGroup: Book
Binding: Hardcover

Astronomy | Astronomy | Science | Subjects | Books

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ASIN: 1400060109
Release Date: 2006-02-14

Book Description

In this riveting book, acclaimed journalist Kathy Sawyer reveals the deepest mysteries of space and some of the most disturbing truths on Earth. The Rock from Mars is the story of how two planets and the spheres of politics and science all collided at the end of the twentieth century.
It began sixteen million years ago. An asteroid crashing into Mars sent fragments flying into space and, eons later, one was pulled by the Earth’s gravity onto an icy wilderness near the southern pole. There, in 1984, a geologist named Roberta Score spotted it, launching it on a roundabout path to fame and controversy.
In its new home at NASA’s Johnson Space Center in Houston, the rock languished on a shelf for nine years, a victim of mistaken identity. Then, in 1993, the geochemist Donald “Duck” Mittlefehldt, unmasked the rock as a Martian meteorite. Before long, specialist Chris Romanek detected signs of once-living organisms on the meteorite. And the obscure rock became a rock star.
But how did nine respected investigators come to make such startling claims about the rock that they triggered one of the most venomous scientific battles in modern memory? The narrative traces the steps that led to this risky move and follows the rippling impact on the scientists’ lives, the future of space exploration, the search for life on Mars, and the struggle to understand the origins of life on Earth.
From the second the story broke in Science magazine in 1996, it spawned waves of excitement, envy, competitive zeal, and calculation. In academia, in government agencies, in laboratories around the world, and even in the Oval Office–where an inquisitive President Clinton had received the news in secret–players of all kinds plotted their next moves. Among them: David McKay, the dynamic geologist associated with the first moon landing, who labored to achieve at long last a second success; Bill Schopf of UCLA, a researcher determined to remain at the top of his field and the first to challenge McKay’s claims; Dan Goldin, the boss of NASA; and Dick Morris, the controversial presidential adviser who wanted to use the story for Clinton’s reelection and unfortunately made sure it ended up in the diary of a $200-an-hour call girl.
Impeccably researched and thrillingly involving, Kathy Sawyer’s The Rock from Mars is an exemplary work of modern nonfiction, a vivid account of the all-too-human high-stakes drive to learn our true place in the cosmic scheme.

Customer Reviews:

Rock from Mars Review.......2007-01-20

This is a great book if you like exploring where science originates and how personal battles control the dominant paradigm. Sawyer traces the journey of ALH84001 from its discovery in the Antartic ice by Robbie Score to its place of fame as the first possible clue to possible bacterial life on Mars. Sawyer also includes the massive controversy at stake, the secrecy of the research, and the rush accompanied with going public with their findings. In addition, she explores the backlash against the claims of the McKay group claims and their attempts to explore every possible avenue of contamination. Bringing together scientists across the board from glacial geologists to chemical specialists, ALH84001 allows almost everyone in the scientific community to evaluate the possibility that Mars might have seeded Earth with microbacteria or vice versa and, thanks to Sawyer, you can too.

Human Reaction In the Face of a Possible Paradigm Shift.......2006-07-13

This book is a page-turner! The possibility of having discovered traces of ancient Martian life, no matter how primitive, has sent ripples throughout the (mainly scientific) world. This book gives an excellent overview of the entire story - from the 1984 discovery of this Martian rock in the Antarctic to the present time. As expected, there was much debate about whether the rock did indeed show signs of primitive, ancient Martian life. Consequently, two main camps formed: those trying to prove that the rock did show such signs of Martian life and those proposing alternative explanations for the rock's interesting features. I think that the author has done an excellent job in presenting the story without taking sides in the occasionally heated debates that took place over the years. There are no good guys and no bad guys here, just people trying to understand what had been found in the face of a possible paradigm shift. This book can be enjoyed by anyone because of its clear prose and engaging writing style. Nevertheless, because of its subject matter, it will likely be more popular among science buffs.

Tales of the Rock Star.......2006-05-12

We are fascinated by the possibility that there may be some sort of life elsewhere than on the Earth. The possibility that there is no life elsewhere is equally interesting, but it doesn't, for instance, make interesting science fiction movies. Life outside of Earth has most often been imagined on Mars, which for all its differences from our planet is the one that is most similar to our own. Thus, when on 7 August 1996 researchers announced that they had found evidence that might show fossilized life on Mars, it was not just a scientific announcement, but one which that non-scientist President Clinton had to take part in making. _The Rock from Mars: A Detective Story on Two Planets_ (Random House) by Kathy Sawyer tells how the announcement came about, the science behind it, and the personalities (and the infighting) that made it happen and have kept research in the arena to the current day at various cutting edges at the limits of our understanding. Sawyer, a science reporter for the _Washington Post_, has made this story not only interesting but exciting, a refreshing view of how big science is done these days.

The story began sixteen million years ago with an asteroid slamming into Mars. This sent up debris, and some of the debris became asteroids in their own right, and came down on Earth. This particular rock came down 13,000 years ago, and remained in the ice of Antarctica until it was discovered in 1984. The special nature of the rock, wasn't understood until 1993, when geochemists started examining it, and found that it was 4.5 billion years old; it was the oldest known rock from any planet including our own. More important, they found carbonates and iron crystals that were similar to such chemicals produced by organisms on Earth. Sawyer carefully explains the process of examining the rock; acid, electron scanning microscopes, ultraviolet lasers and more are brought upon it. There is lots of evidence that was turned up, and whatever the aftermath of the research, the team of David McKay, a famously careful and conservative researcher, did such a thorough job that the evidence was never in question. It was the interpretation of the evidence that proved to be troubling. Many scientists were upset that the researchers were taking undue advantage of a hot story and making it seem that their interpretation was factual rather than tentative. NASA was criticized from the start for hyping the research and using it for political reasons. In the ten years since the announcement, the controversy has become less prominent, but among scientists who are looking into the subject, there are still opposing camps on the matter, and vehement disagreements, and hurt feelings.

As Sawyer winds up the story, there is no overall agreement on just what McKay's team turned up. There have been different ways of looking at the rock since then, none of them making a conclusive case. This is not a bad thing. Because of the controversy, new techniques have been brought into play and new discoveries have been made. For instance, what was learned about possible earthly contamination of the rock will be used when bits of Mars are brought back by robot spacecraft sometime in the future. Because of the controversy, there has been increased interest and better explanations for the origins of life on Earth in the most unlikely and unwelcoming of environments. With its depiction of all-too-human scientists attempting objectivity when contemplating the great mystery of life elsewhere, Sawyer's account is an appealing picture of a good example of how science works.

Science vs Politics. (Guess who wins?).......2006-04-01

Kathy Sawyer does an absolutely first-rate job of describing what is really a very intricate subject--what is life, and how do we know? The first half of the book describes the discovery of a meteor lying on the snow in Antarctica--which turns out of have been ejected from the planet Mars! The initial investigation of these rocks is cursory and tells little that is new. The rock molders in a museum repository for years--until it is examined again. Suddenly, in a leap of inspiration, one scientist notices tiny features that look strikingly like fossilized microbes--the first signs of extraterrestrial Life!

President Clinton announces the discovery, and the second half of the book describes the intense politicking that goes on as scientists jockey furiously for air time to claim credit for or denounce the sensational discovery. Few books give a clearer picture of the rampant egotism that dominates science just as much as it dominates every other field of human endeavor. So much for the vaunted impartiality of the "scientific mind." (Indeed, please find me a single left-wing scientist who disagrees that humans cause global warming--or a single conservative scientist who thinks they do!)

Why not five stars for this terrific book? Well it is a fine coda to what is surely the best book on extraterrestrial life "Rare Earth: Why Complex Life in Uncommon in the Universe." That's Five Stars worth of reading. (Read it first, and then you'll really enjoy "The Rock From Mars.")

A Story of Big Science.......2006-03-16

From movies and television the public has an image of the scientist being a selfless, mild mannered, seeker of knowledge. 'Taint so.

Scientists are people just like the rest of us. They are competitive with each other and with the world at large. They establish theories and points of view that they will defend almost to the death. When an alternative view comes around there is not the dispassionate scientific openness that allows honest discussion. Instead there is a very passionate series of thoughts centered around what this will do to the grants and funding that that scientist has. With that comes money, status, grad students -- all the things that matter most to a scientist.

This is the story of a rock found in Antarctica. First it was just a rock. Then it became clear that it came from Mars. (The evidence is well developed in the book.) Then they spotted things that might indicate that there was or had been life on Mars. Then it hit the fan.

Life anywhere but Earth has all kinds of meanings (for instance to the churches - intelligent design and all that). There could be entirely new branches of biology. The story of proving that this was or was not evidence of life on Mars fills the rest of the book. It was a vicious fight. It's a supurb book.

Was there life on Mars? We really don't know. Even with all the space craft that have visited Mars, including the two rovers, we really don't know.

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Great plot, awful politics
Good Concept, Bad Politics
This was a very Entertaining Read for someone who likes Tech
Political Sci-Fi
"Story and Characters Plod Along"

Life Probe
Michael Mccollum
Manufacturer: Del Rey
ProductGroup: Book
Binding: Mass Market Paperback

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ASIN: 0345302958
Release Date: 1983-05-12

Book Description

Trade paperback version of science fiction novel. Originally published by Del Rey. Book is large format, printed on bright white paper, perfect bound, with full color, plastic laminated covers. Book is autographed by the author.

Customer Reviews:

Great plot, awful politics.......2006-11-30

I read this book in middle school and loved it to pieces. I recently reread it as someone with a PhD in political science. The pronounced ethnocentrism and black-helicopter UN-phobia is jarring to me now, but the story is still compelling. In the end, the best character in the book is an inanimate probe. The book sets up nicely for a sequel, but is also a self-contained, intriguing story about a cry for help from a doomed race.

Good Concept, Bad Politics.......2003-09-26

The concept is good enough, though a little flawed. I mean a super advanced civilization, who have been spacefaring even before the Neanderthals roamed the Earth, thinks itself "doomed" because they have not yet discovered Faster Than Light travel? Their solution: send sublight probes everywhere to search for some other civilization that has FTL. Takes tens of thousands if not hundreds of thousands of years with this approach to "save their civilization". In the story this civilization has already used "slowboats" or generation ships even before they started using sublight interstellar exploratory sentient robot probes. Now they are not REALLY doomed even without FTL. Once their system's resources are all out, or almost all out, they can just put their ENTIRE population on as many slowboats as they need (and believe me, they have the capability to make a lot of ships!) and transplant themselves to some new, uninhabited, full-of-natural-resources brand new star system!

Now about the politics....the author is obviously caucasian, and grew up in the west. He pretends to understand the struggles of those referred to in the novel as "black and brown" people, but he doesn't. He pretends to understand what effects British and Dutch colonization had on them, but doesn't. He pretends to understand their views but doesn't. Instead he portrays them as warmongering greedy people out to start a world war, when (even in his own futuristic story), many of them are still opressed or not treated as equals, even with the re-organized-more-equal (supposedly)-UN ruled world.

My advise to the author. Stick to sci-fi, don't insert politics into your novels. The bad politics just messes up the good sci-fi.

This was a very Entertaining Read for someone who likes Tech.......2001-12-18

There was enough of a Story to pull it along nicely.
I was captivated by the Romance of a Life Probe / A.I. struggling to "Continue Existing" despite HORRENDOUS damage.

The majority of the Life Probe was damaged.

Part of the probe continued its existence in an A.I. form that was CREATED by the Original Life Probe. This form allowed the Human Community to interact with it Much like a human, and actually we freely adopted its technologies that it was based on, with it's own intelligent assistance. Note, that the ORIGINAL purpose of the Life Probe A.I. was lost with the immense damage that it took. The only part of the "probe" that remained was its experimental "Human Model" that it (the much more intelligent and ALIEN life probe ENTIRE) was going to use to study/communicate with the Humans.

After the probe was damaged, We humans were able to freely investigate it(since it could not defend itself). We could freely speculate on it's origins and use the technology that it was based on to further our OWN development and purposes.

A very interesting fantasy.
A very intelligent & powerful machine that fell prey to circumstance, and became OUR unwitting "benefactor" And a source of new technology in more ways than one.

I'm re-purchasing the book b/c I read it in the 80's and thought it was excellent.

Political Sci-Fi.......2001-11-16

Although the writing and character development are generally lackluster, what makes Life Probe an intriguing novel is the political angle. Written during the Cold War, Life Probe shows a remarkable ability to think beyond the US-Russia framework and envision a world afterward. Like many authors, McCollum hypothesizes that a nuclear war in the near future leads to the development of a functioning United Nations. In this context, North-South political wrangling in the 21st century underlies much of the book. The book is somewhat right-wing, in that a "Star Wars"-type defense system is depicted as being highly successful in preventing much of the damage from the near-future nuclear war, and the discussion of the North-South conflict is strongly biased against the South. Nevertheless, McCollum does discuss the issues and makes a reasonable case for both sides--which impressed me. Two other aspects of the book are well worth mentioning: (1) the alien spacecraft's view of Earth and its attempts to manipulate Earth politics are well depicted, and (2) the discussion of science hits just the right balance between the technical and the descriptive. The political wrangling never leads to a real moral dilemma for the reader, since you always know who to root for, and the picture of parliamentary maneuvering is naive--but the book is charming, balanced, supported by interesting ideas, and has enough twists and turns to keep a reader happy. I recommend it as a good light read. On balance, I think it's McCollum's best novel.

"Story and Characters Plod Along".......2000-08-07

Seventy pages into it and I put it down. I admit, the scenes with the probe and its thoughts were pretty neat. But when it came to the human characters and their efforts to move the story forward it fell flat. The probe shows up as a mysterious glow in space. While the UN launches an investigation, that seems to take a back seat to other subplots that failed to grab my interest.

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Molecular Methods for Virus Detection

Manufacturer: Academic Press
ProductGroup: Book
Binding: Spiral-bound

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Book Description

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.
Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.

Key Features
* Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions
* Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT
* Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence
* Includes quality control information crucial in research and clinical laboratories
* Most chapters are written by the inventors and principal developers of the methodologies
* Includes color plates, 77 figures, and 18 tables

How to Dissect: Exploring With Probe and Scalpel

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How To Dissect
An amazing book that shows the internal anatomy in detail
This book should be made a compulsory school text!

How to Dissect: Exploring With Probe and Scalpel
William Berman
Manufacturer: Prentice Hall
ProductGroup: Book
Binding: Hardcover

ASIN: 0668059397

Customer Reviews:

How To Dissect.......2003-05-24

I used this book in my homeschool dissection club and found that it is a good reference tool, but lacks a thorough description of the internal anatomy. The diagrams of the internal anatomy are also very vague. This book is best used with online dissecton diagrams and instuctions. In a nutshell, this book came in handy every time I dissected, but only after I had a complete understanding of the internal anatomy of the specimen from other sources.

An amazing book that shows the internal anatomy in detail.......1999-05-28

I have been looking for a book about dissection for many years. Now I have found one. This book is graet. Besides have detailed discription on dissections it also has many experiment to go with the dissection. This book is fantastic!!!!!!!!!!!!!!!!!!!!!!!!

This book should be made a compulsory school text!.......1998-09-02

I am a privileged owner of the 1961 edition of this book, having bought it in a jumble sale in 1984 for a mere Singapore 70 cents (less for U.S.). Open-ended & with great science projects, this book defies obsolescence and is a must for any parent/nation committed in honing young minds into the field of experimental biology and science.

With this book, William Berman leaves a legacy which plays surrogate to any young inquiring mind wishing for the most inspiring biology teacher.

Book Description

The dangers and drawbacks inherent in radioactivity-based methods along with a demonstrated and dramatic increase in sensitivity have precipitated a major shift towards luminescence measurements and visualization techniques. Their use has now spread even to traditional clinical environments, and their applications have grown from clinical assays to DNA sequencing, antioxidant detection, and high-throughput screening. Luminescence Biotechnology: Instruments and Applications furnishes a thorough w review of the principles and applications of luminescence. With a consistent focus on practical considerations, contributions from a team of internationally acclaimed authors take you from the fundamentals of the different luminescence-based assay systems, calculation methods, and instruments through the spectrum of applications and latest research advances. Topics include gene and protein assays, oxidative stress and tissue aging, applications of luminescent microspheres, and proton image analysis. This book clearly identifies the advantages of luminescence over other assay techniques, discusses its potential pitfalls, and illustrates the broad range of its utility. Whether you are a newcomer to the field or a seasoned professional, this book provides a wealth of information that will bring you quickly up to date on the technology, recent research developments, and cutting-edge applications.

Fluorescent and Luminescent Probes (Biological Techniques Series)

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Fluorescent and Luminescent Probes (Biological Techniques Series)

Manufacturer: Academic Press
ProductGroup: Book
Binding: Spiral-bound

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Principles of Fluorescence Spectroscopy

ASIN: 0124478360

Book Description

The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.
The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.

TOPICS COVERED:
* Single and multiphoton confocal microscopy
* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies
* Applications of new optical probes for imaging proteins in gels
* Probes and detection technologies for imaging membrane potential in live cells
* Use of optical probes to detect microorganisms
* Raman and confocal raman microspectroscopy
* Fluorescence lifetime imaging microscopy
* Digital CCD cameras and their application in biological microscopy

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