Hoffmann-La Roche Inc., Nutley, NJ. Describes methods in detail, ranging from traditional affinity purification methods as immunoaffinity chromatography, to the use phage-display technology in the discovery of affinity ligands and drugs. For researchers.
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Protein'Protein Interactions: Methods and Applications (Methods in Molecular Biology)
Manufacturer: Humana Press
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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition
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Molecular Cloning: A Laboratory Manual (3-Volume Set)
ASIN: 1588291200 |
Book Description
A collection of highly successful biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions. These readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches, such as the Scansite program for computational analysis. These powerful tools are essential for deciphering how proteins interact with each other to form biological networks, as well as for unraveling protein-protein interactions in disease to find novel therapeutic targets.
Book Description
Easy to read and up-to-date, this guide will help both first-time writers and experienced contributors in authoring research papers. Although the examples are extracted mainly from the medical and biological sciences, the principles described apply to virtually every branch of science. The book provides step-by-step information on how to prepare every aspect of a scientific paper and examples of both good and bad writing illuminate the author's advice in this accessible and informative handbook.
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Teacher and Engineer.......2004-12-06
This book is ok for the beginner or undergradute student. The materil is not very detailed. It is good for the introduction for people who is usign Egnlish as a secound languge.
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Lipase and Phospholipase Protocols (Methods in Molecular Biology)
Manufacturer: Humana Press
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Binding: Hardcover
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ASIN: 0896035468 |
Book Description
A unique collection of step-by-step methods for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of unique substrates for the determination of lipolytic activity, the isolation of purified lipase preparations, and the development of high expressing recombinant systems. There are also methods for the immunodectection and production of immunological reagents and the analysis of biochemical properties, such as subunit size, lipase kinetics, enzyme immobilization, and ligand interactions. The protocols covers more than a dozen specific enzymes, and provide well-established methods that can be adapted to the discovery of new lipase enzymes.
Book Description
The science and practice of medicine has undergone a fundamental change as a result of large-scale genome projects that led to the sequencing of a number of important microbial, plant and animal genomes in the last 5 years. This book aims to combine industry standard software engineering and design principles, genomics and bioinformatics and cancer research. It focuses on creating and integrating practical, useful tools for the scientific community in the context of real-life, real-value biomedical problems that researchers face on a routine basis, rather than being just a didactic exercise in learning a programming platform. The book leverages technologies for molecular biology, genomics and bioinformatics and cancer research developed by the NIH, NCI-Center for Bioinformatics (NCICB), the National Center for Biotechnology Information (NCBI, a division of the National Library of Medicine (NLM) at the NIH) and Stanford University.
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Diagnostic Techniques in Genetics
Manufacturer: Wiley
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Binding: Paperback
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Color Atlas Of Genetics (Flexibook)
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Molecular Diagnostics
ASIN: 0470870257 |
Book Description
Recent developments within molecular biology and genetic engineering have led to huge advances and changes within the biological sciences especially within the field of human genetics.
Diagnostic Techniques in Genetics offers an important overview of how DNA or RNA technology may be applied to a large set of genetic diagnoses.
The first part of the book focuses on DNA/RNA applications and includes many of the latest developments in the field combined with routine procedures of genetic diagnoses, for example cloning and sequencing DNA. The DNA applications presented in the first chapter are then each applied to a specific kind of genetic diagnosis and the text concludes with a chapter devoted to population genetics.
First published in French by Dunod in 2002, this book is an excellent reference for students taking courses in molecular biology, medicine and medical genetics. It is also a useful introduction for postgraduate students and researchers in the field who require a general overview of genetic diagnoses.
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