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Manipulating the Mouse Embryo: A Laboratory Manual
Manufacturer: Cold Spring Harbor Laboratory Press
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Binding: Paperback
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Atlas of Mouse Development
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ASIN: 0879695919 |
Book Description
Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.
Customer Reviews:
The New Testament?.......2004-01-20
A definitive summary of mouse development and simple, concise proven protocols. This is the best publication there is for this kind of work. It's much better than the 2nd, ed. I wish it had more of a troubleshooting focus, but I guess if you are in this line of work, you should be able to figure out problems yourself, or query the tg list.... I like how there are alternative strategies mentioned depending on how your lab is equiped (or not).
Book Description
The most complete resource on the techniques, equipment,principles, and practices of animal cell culture
Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.
The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites
Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Customer Reviews:
Fantastic book!.......2007-09-08
Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.
useful book.......2007-08-09
There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.
Save on Textbook.......2006-08-15
I purchased this book with a description of like new. I would say this is new. The previous person who used this did not even break in the binder. This is an excellent reference book, I have enjoyed reading the text up to this point.
Best Basic Book on Tisssue Culture.......2006-07-07
For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.
The best book on cell culture.......2005-01-09
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
Book Description
Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations.
The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research
Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level
Expanded to 2 volumes.
Customer Reviews:
no longer short.......2006-12-05
I used a previous edition of this book in graduate school, and it was an excellent resource. Covering a broad range of protocols in a complete but concise manner, it was a single albeit fat volume that was portable and reasonably priced. In comparison to Maniatis and the full version of CP, this edition remains less extensive and less expensive, however in expanding to 2 volumes it has lost the advantage of portability that was (what I thought) really set it apart from others. The price of this edition has also increased to the point where the value of owning a personal copy has become highly questionable.
an excellent brief reference book.......2003-04-12
This is a well-organized, clear, short reference work. Well done
The (little) Red book..........2002-08-26
Here is the little red bok.
If the big one is too expensive for you, you can always buy this. You'll find inside all the important protocols and data for molecular biology.It's up to date, and clearly presented.
Try it, and then buy the big one!
A very good reference manual.......2000-05-27
This book is an essential tool for people in the scientific field such as Molecular Biology (obviously), Biochemistry, and Neuroscience. It is comprehensive and up-to-date as far as the techniques are concerned. It is good value in a sense that you don't have to buy the whole "Current Protocols Series" which costs an arm and a leg if you do. Although nowadays, a lot of "kits" are commercially available, the techniques found in this book explain principles and provide different alternatives suited for your needs. Molecular Cloning by Maniatis et al., although needs updating, is still a helpful reference in my opinion and it complements this book.
A Must-Have for any Molecular Biologist..........2000-03-17
I originally bought this book as a secondary source of information (Maniatis has ben my bible now for a few years...) but as time has progressed I have found myself using this book more and more. The protocols are easy to follow, logically placed, give enough information so you understand what it is you are doing, are up to date, and are all in one volume. Yes, this has effectively replaced my copy of Molecular Cloning (which is in desperate need of an update). Great job!
Book Description
KEY MESSAGE: Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case.
Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms; Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown; Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria; Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods; Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms; Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing; Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens; The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration of Bacteriophages, Plant Viruses; Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique; Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample; Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton; Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria.
For all readers interested in microbiology.
Customer Reviews:
Great.......2006-11-08
I recieved my book in a fast time frame and it was exactly what I needed for school!
Lab Manual.......2006-02-23
Great manual for the laboratory... Lack of pictures is the main detraction from this product. Only a few pages of color photos.
Book Description
The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.
Customer Reviews:
IT COVERED BOTH TECHNIQUES AND PRECAUTIONS.......2003-03-30
Apart from hinting that this book is more expensive than I had expected, there is no negative comment I have for it.
It is a well-defined lab manual, which will be useful for both students and investigative scientists. The book covered all aspect of microbiology: including related parasitology topics.
It explained various methods used in the identification and classification of all types of microorganisms. High-level undergraduates should appreciate the directives it gave concerning culture-media preparation, as well as sequential isolation of bacteria, fungi, viruses, protozoa, and microscopic algae. The same applies to its handling of general microscopy: including the advantages and disadvantages of each type of microscope. This is a very fine companion, which discussed every technique and precaution used in contemporary microbiology lab.
La microbiologia.......2002-05-08
La microbiología es la rama de la biología más interesante, conocer el metabolismo y forma de vida de las bacterias va más halla de lo que uno puede imaginar, cada una tiene su propio nombre y caracteristicas que las distinguen unas de otras. A mi parecer las enterobacterias es lo más emocionante que puede existir en esta área, su identificación es una tarea entretenida y muy emocionante. La microbiología es lo máximo.
Customer Reviews:
Lab Guide.......2005-10-03
The lab guide is very helpful for the lab. It was very specific and detailed.
An excellent manual for teaching decriptive embryology.......2001-05-02
I witnessed the birth of the first edition of this book when I was still a college student, and have seen its transformation to suit the needs of each new era. The book offers fundamental principles in descriptive embryology. I hoped to see more on the expperimental aspect of the subject. I also wished to see the embryology of more of the animal phyla (eg. fruit fly, zebra fish) included.
An Embryology Lab Instructor's Comments.......2000-04-28
I have used this book in my course for four years. Typically one-hundred students per year are enrolled in my course. The quality of the photomicrographs is excellent. My only criticisms of the micrographs are that 1.) The sequence of the photomicrographs would be enhanced if they were arranged in a developmental sequence. Presently, the student must flip pages constantly to view all slides relevant to a particular developmental stage. 2.) There are not enough sagittal sections of the chick embryos. 3.) 96 hour chick embryos, instead of pig embryos might be an improvement. The text is good, although not quite as thorough as the Matthews lab manual was.
Average customer rating:
- Micro lab book
- Microbiology Lab Manual
- A good beginner's manual
- great
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Microbiology: A Laboratory Manual (7th Edition)
James Cappuccino , and
Natalie Sherman
Manufacturer: Benjamin Cummings
ProductGroup: Book
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ASIN: 080532836X |
Book Description
This versatile laboratory manual can be used with any undergraduate microbiology text and course. Known for its brief laboratory activities, minimal equipment requirements, and competitive price, the manual includes a variety of experiments selected to assist in the teaching of basic principles and techniques. Each of the 77 experiments includes an overview, a purpose, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Comprehensive introductory material and laboratory safety instructions are provided. For college instructors and students.
Customer Reviews:
Micro lab book.......2007-08-09
I bought this book because it was required for my microbiology lab. I hated this class to begin with but by then end i LOVED IT! The book is so helpful and easy to learn from. I would reccommend it to anyone.
Microbiology Lab Manual.......2007-02-10
This book arrived in excellent condition within the specified time. It was a brand new book and was exactly as advertised.
A good beginner's manual.......2006-03-10
This book is very clear and straight-forward. If you read the text ahead of time, you will clearly understand the procedures and theoretical context for your lab class. It clearly is a beginner's manual, though, so it does not go terribly in depth into the molecular biochemistry of the experiments, which for many people will be a good thing, but not for those who want to learn everything about everything.
great.......2005-09-27
I was very please. Service was fast, price awedsome,and merchandise in perfect shape. I will keep buying from you guys!
Book Description
Short Protocols in Immunology is an easy-to use companion to Current Protocols in Immunology. Drawn from the most fundamental and widely used methods in the well-known parent volume, this book provides step-by-step protocols in their most easily accessible format for use at the bench. Immunologists in basic science and clinical work alike will find techniques they rely on, including production, purification, and fragmentation of antibodies; assays for lymphocyte function; measurement of cytokines and cytokine receptors; biochemistry of lymphocyte activation; innate immunity, isolation of human immune cell populations, detection and analysis of HIV, and animal models for a variety of autoimmune and infectious diseases.
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