Molecular Fluorescence: Principles and Applications

Book Description

Today, fluorescence spectroscopy is an important tool of investigation in many areas. In analytical sciences, its advantage is extremely high sensitivity and selectivity - even single molecules can be detected - and it achieves a high spatial resolution and time resolution in combination with microscopic techniques or laser techniques, respectively. In material sciences, this is used to study structure and dynamics of surfaces. Particularly in the areas of biochemistry and molecular genetics, fluorescence spectroscopy has become a dominating technique. Together with the latest imaging techniques, fluorescence spectroscopy allows a real-time observation of the dynamics of intact biological systems with an unprecedented resolution.
This book offers a comprehensive introduction to and survey of fluorescence spectroscopy. It is written for newcomers and active researchers alike who are learning to apply fluorescence methods in the areas of chemistry, physical chemistry, polymers, materials, colloids, biochemistry, biology, medical and pharmaceutical research.

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great for beginners, but too few references.......2003-12-07

This is a great introductory book. Beautifully edited, very well written, with a lot of information to start digging each subject; I just missed a few more references in each chapter, and also more references to journals and not just other books, and then I would have been a 5 star.

NMR for Physical and Biological Scientists

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NMR for Physical and Biological Scientists
Thoma Pochapsky
Manufacturer: Garland Science
ProductGroup: Book
Binding: Hardcover

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ASIN: 0815341032

Book Description

Nuclear Magnetic Resonance spectroscopy is a dynamic way for scientists of all kinds to investigate the physical, chemical, and biological properties of matter. Its many applications make it a versatile tool previously subject to monolithic treatment in reference-style texts. Based on a course taught for over ten years at Brandeis University, this is the first textbook on NMR spectroscopy for a one-semester course or self-instruction. In keeping with the authors' efforts to make it a useful textbook, they have included problems at the end of each chapter.

The book not only covers the latest developments in the field, such as GOESY (Gradient Enhanced Overhauser Spectroscopy) and multidimensional NMR, but includes practical examples using real spectra and associated problem sets. Assuming the reader has a background of chemistry, physics and calculus, this textbook will be ideal for graduate students in chemistry and biochemistry, as well as biology, physics, and biophysics. NMR for Physical and Biological Scientists will also be useful to medical schools, research facilities, and the many chemical, pharmaceutical, and biotech firms that offer in-house instruction on NMR spectroscopy.

Spectroscopy for the Biological Sciences

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Spectroscopy for the Biological Sciences
Gordon G. Hammes
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Paperback

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ASIN: 0471713449

Book Description

An introduction to the physical principles of spectroscopy and their applications to the biological sciences

Advances in such fields as proteomics and genomics place new demands on students and professionals to be able to apply quantitative concepts to the biological phenomena that they are studying. Spectroscopy for the Biological Sciences provides students and professionals with a working knowledge of the physical chemical aspects of spectroscopy, along with their applications to important biological problems.

Designed as a companion to Professor Hammes's Thermodynamics and Kinetics for the Biological Sciences, this approachable yet thorough text covers the basic principles of spectroscopy, including:
* Fundamentals of spectroscopy
* Electronic spectra
* Circular dichroism and optical rotary dispersion
* Vibration in macromolecules (IR, Raman, etc.)
* Magnetic resonance
* X-ray crystallography
* Mass spectrometry

With a minimum of mathematics and a strong focus on applications to biology, this book will prepare current and future professionals to better understand the quantitative interpretation of biological phenomena and to utilize these tools in their work.

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An introduction to the physical principles of spectroscopy and their applications to the biological sciences Advances in such fields as proteomics and genomics place new demands on students and professionals to be able to apply quantitative concepts to the biological phenomena that they are studying. Spectroscopy for the Biological Sciences provides students and professionals with a working knowledge of the physical chemical aspects of spectroscopy, along with their applications to important biological problems. Designed as a companion to Professor Hammes's Thermodynamics and Kinetics for the Biological Sciences, this approachable yet thorough text covers the basic principles of spectroscopy, including: Fundamentals of spectroscopy Electronic spectra Circular dichroism and optical rotary dispersion Vibration in macromolecules (IR, Raman, etc.) Magnetic resonance X-ray crystallography Mass spectrometry With a minimum of mathematics and a strong focus on applications to biology, this book will prepare current and future professionals to better understand the quantitative interpretation of biological phenomena and to utilize these tools in their work.

Trace Element Analysis in Biological Specimens (Techniques and Instrumentation in Analytical Chemistry)

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Trace Element Analysis in Biological Specimens (Techniques and Instrumentation in Analytical Chemistry)

Manufacturer: Elsevier Science
ProductGroup: Book
Binding: Hardcover

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ASIN: 0444898670

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The major theme of this book is analytical approaches to trace metal and speciation analysis in biological specimens. The emphasis is on the reliable determination of a number of toxicologically and environmentally important metals. It is essentially a handbook based on the practical experience of each individual author. The scope ranges from sampling and sample preparation to the application of various modern and well-documented methods, including quality assessment and control and statistical treatment of data. Practical advice on avoiding sample contamination is included.

In the first part, the reader is offered an introduction into the basic principles and methods, starting with sampling, sample storage and sample treatment, with the emphasis on sample decomposition. This is followed by a description of the potential of atomic absorption spectrometry, atomic emission spectrometry, voltammetry, neutron activation analysis, isotope dilution analysis, and the possibilities for metal speciation in biological specimens. Quality control and all approaches to achieve reliable data are treated in chapters about interlaboratory and intralaboratory surveys and reference methods, reference materials and statistics and data evaluation.

The chapters of the second part provide detailed information on the analysis of thirteen trace metals in the most important biological specimens. The following metals are treated in great detail: Aluminium, arsenic, cadmium, chromium, copper, lead, selenium, manganese, nickel, mercury, thallium, vanadium and zinc.

Book Description

Tools, techniques, and progress in cancer biomarkers discovery

The completion of a number of gene sequencing projects, recent advances in genomic and proteomic technologies, and the availability of powerful bioinformatics tools have led to promising new avenues and approaches in the search for cancer biomarkers. This book provides a comprehensive overview of current methodologies and technologies. It discusses biomarker discovery as a whole, rather than focusing on one specific marker or cancer. With information on both existing and potential biomarkers, Cancer Biomarkers: Analytical Techniques for Discovery:
* Provides insights into the current technological platforms for biomarker discovery, including mass spectrometry combined with multidimensional chromatography, DIGE, and various chip technologies
* Includes a detailed discussion of protein networks and protein phosphorylation in cancer
* Details the use of imaging mass spectrometry, laser capture microdissection, serial analysis of gene expression, enzyme-linked immunosorbent assays, protein microarrays, antibody-based microarrays, and bioinformatics
* Covers the emerging role of surface-enhanced laser desorption ionization (SELDI) and various tagging and labeling strategies
* Discusses related regulatory and ethical issues

With a wealth of information that can be applied to a broad spectrum of biomarker research projects, this is a core reference for biomarker researchers, scientists working in proteomics and bioinformatics, pharmaceutical scientists, oncologists, biochemists, biologists, and chemists.

In Vivo EPR (ESR): Theory & Applications (Biological Magnetic Resonance)

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In Vivo EPR (ESR): Theory & Applications (Biological Magnetic Resonance)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 0306477904

Book Description

In Vivo EPR (ESR) is a textbook on this relatively new subject in biomedical electron spin resonance. While a few chapters have appeared in special topics volumes in this series, this book covers the principles and theory, instrumentation as well as the latest applications at the time of its writing. The authors are world-renowned experts and pioneers in their fields. This book is divided into two major sections dealing with theory and instrumentation, and aspects of biochemistry, in vitro and in vivo applications. A significant amount of detail is devoted to clinical applications and the problems and pitfalls encountered in in vivo spectroscopy and imaging.
Key Features:

-History of In Vivo EPR,
-Principles of Imaging-Theory and Instrumentation,
-Time-domain Radio Frequency EPR Imaging,
-The Measurement of Oxygen In Vivo Using In Vivo EPR Techniques,
-Potential Medical (Clinical) Applications of EPR,
-Combining NMR and EPR/ESR for In Vivo Experiments.

Infrared and Raman Spectroscopy of Biological Materials (Practical Spectroscopy)

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Infrared and Raman Spectroscopy of Biological Materials (Practical Spectroscopy)
Hans-Ulrich Gremlich , and Bing Yan
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 0824704096

Book Description

Infrared and Raman Spectroscopy of Biological Materials facilitates a comprehensive and through understanding of the latest developments in vibrational spectroscopy. It contains explains key breakthroughs in the methodologies and techniques for infrared, near-infrared, and Raman spectroscopy. Topics include qualitative and quantitative analysis, biomedical applications, vibrational studies of enzymatic catalysis, and chemometrics.

Very High Frequency (VHF) ESR/EPR (Biological Magnetic Resonance)

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Very High Frequency (VHF) ESR/EPR (Biological Magnetic Resonance)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 0306483165

Book Description

Very High Frequency (VHF) ESR/EPR focuses on a new area of electron spin resonance spectroscopy that has blossomed during the past decade. This textbook both highlights the state-of-the-art of this field while covering the full scope of VHF EPR in a single volume. The topics were chosen to cover areas in which VHF EPR seems to be especially productive, as well as critical new technical developments. The contributors of the book are leaders in their fields. They emphasize the need for VHF EPR, describe results that could only be achieved with VHF EPR, and discuss the further potential of this evolving field. This volume provides information that will be useful for many years, focusing on what advantages and benefits one may expect from VHF EPR.
Key Features:
-History of Very High Frequency EPR,
-Principles of High-Field EPR, Instrumentation and Resonators for Quasioptical EPR,
-Applications to Photosynthesis, Protein Structure, Molecular Dynamics, Metal Sites in Proteins, Transition Metal Centers, Small Inorganic Models and Spin Labels in Membranes,
-Pulse High Frequency EPR, Time-Resolved High Frequency and Multifrequency EPR,
-High Frequency ESEEM and ENDOR.

ESR Spectroscopy in Membrane Biophysics (Biological Magnetic Resonance)

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ESR Spectroscopy in Membrane Biophysics (Biological Magnetic Resonance)
Marcus A. Hemminga , and Lawrence J. Berliner
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 0387250662

Book Description

Spectroscopic methods are not only important as an analytical tool, they also provide information about fundamental physical and chemical properties of molecules, the molecular and electronic structure, and the dynamic behaviour of molecules. Starting from a comprehensive quantum mechanical description, ESR Spectroscopy in Membrane Biophysics introduces the optical (IR, Raman, UV/Vis, CD, fluorescence and laser spectroscopy) and magnetic resonance (1D and 2D-NMR, ESR) techniques.

ESR Spectroscopy in Membrane Biophysics is a timely review of the increasing interest in using spin-label ESR as an alternative structural technique for NMR or X-ray diffraction. It is aimed at training an audience to learn ESR spectroscopy to determine membrane protein structures, conformational dynamics and protein-lipid interaction.

Methods in Enzymology, Volume 402: Biological Mass Spectrometry

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Methods in Enzymology, Volume 402: Biological Mass Spectrometry

Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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Methods in Enzymology, Volume 405: Mass Spectrometry: Modified Proteins and Glycoconjugates

ASIN: 0121828077

Book Description

This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology.

It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. Finally it describes atto-to-zepto-mole quantitation of 14C and 3H by accelerator mass spectrometry.

*Part 1 of 2 volumes about Mass Spectrometry
*Authoritative and comprehensive treatment of protein mass spectrometry in human cell biology
*Presents fundamentals, techniques, instrumentation and bioinformatics
*Provides an overview of proteomics, protein-protein and protein-ligand binding, and biophysical studies

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