Culture of Animal Cells: A Manual of Basic Technique

Book Description

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

Customer Reviews:

Fantastic book!.......2007-09-08

Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.

useful book.......2007-08-09

There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.

Save on Textbook.......2006-08-15

I purchased this book with a description of like new. I would say this is new. The previous person who used this did not even break in the binder. This is an excellent reference book, I have enjoyed reading the text up to this point.

Best Basic Book on Tisssue Culture.......2006-07-07

For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.

The best book on cell culture.......2005-01-09

Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.

Practical HPLC Method Development, 2nd Edition

Average customer rating:

A great book - information overload
Plactical and Rational method Development!
an excellent book on pratical HPLC

Practical HPLC Method Development, 2nd Edition
Lloyd R. Snyder , Joseph J. Kirkland , and Joseph L. Glajch
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 047100703X

Book Description

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Customer Reviews:

A great book - information overload.......2002-07-16

This is a very useful reference to have if you work extensively on HPLC. I find its of more use to understand concepts ...there is a lot of information here.

Which means - if you are looking for a solution to a problem and you want to research separation theory - a great book.

If your looking for a quick fix to an HPLC problem - not as good. This is a detailed, extensive and well written text on HPLC Method development - wish there was more on validation here though.

Plactical and Rational method Development!.......2000-03-15

Rational Method development for HPLC is resented. Instruction of rational development of 3-solvent separation system is excellent. This book also has useful know-hows and tools in practical laboratory experiments, such as strength of hydrogen bonding of base silica and nomograph for estimation of "solvent strength" for reversed phase HPLC.

an excellent book on pratical HPLC.......1998-01-20

The book is useful for gerenal operator who are both novice and expert. It do is pratice. It's better give another chapter for troubleshooting and maintence.

The ELISA Guidebook (Methods in Molecular Biology)

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The ELISA Guidebook (Methods in Molecular Biology)
John R. Crowther
Manufacturer: Humana Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0896037282

Book Description

John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

Average customer rating:

Good student text

Organic Laboratory Techniques
Ralph J. Fessenden , Joan S. Fessenden , and Jess Feist
Manufacturer: Brooks Cole
ProductGroup: Book
Binding: Paperback

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ASIN: 0534379818

Book Description

This highly effective and practical manual is designed to be used as a supplementary text for the organic chemistry laboratory course - and with virtually any main text - in which experiments are supplied by the instructor or in which the students work independently. Each technique contains a brief theoretical discussion. Steps used in each technique, along with common problems that might arise. These respected and renowned authors include supplemental or related procedures, suggested experiments, and suggested readings for many of the techniques. Additionally, each chapter ends with a set of study problems that primarily stress the practical aspects of each technique, and microscale techniques are included throughout the text, as appropriate. Additional exercises, reference material, and quizzes are available online.

Customer Reviews:

Good student text.......2000-06-28

The authors give a nice presentation of theory involved in recrystallization, distillation, chromatography, and extraction. The writing style is academic, but friendly enough to keep you interested. I found it great to read before doing the scheduled experiments in O-Chem lab. However, this is a very "student"-ish text and I seriously doubt professionals would find it useful, unless you're looking for a refresher course in the basic techniques. The labs I took had a significant amount of NMR involved; not much discussion in this text about that. The problems provided are usually good, but there are no answers given. I'd also recommend "Principles and Techniques in an Integrated Chem Laboratory" by Aikens, Bailey, et al.

Oxidative Stress and Antioxidant Protocols (Methods in Molecular Biology)

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Oxidative Stress and Antioxidant Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Free Radical and Antioxidant Protocols (Methods in Molecular Biology)

Accessories:

ASIN: 0896038505

Book Description

Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Clinical Immunology and Serology: A Laboratory Perspective

Average customer rating:

Meh
Clear as mud.

Clinical Immunology and Serology: A Laboratory Perspective
Christine Dorresteyn Stevens
Manufacturer: F. A. Davis Company
ProductGroup: Book
Binding: Paperback

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ASIN: 0803610955

Customer Reviews:

Meh.......2006-12-20

The book is good in that it explains certain basic immunological techniques quite well in a descriptive sense (at least at the molecular leve), however graphically many things are left to be desired. Many topics like explaining detailed immunofluorescent or protein immunology, etc are woefully inadequate for any practical use. The first third of the book explaining immunological processes is hopeless and best left to a dedicated immunology textbook like Janeway or Abbas.

Clear as mud........2006-02-12

This book couldn't decide what it wanted to be. There were sections that were very detailed and not clearly explained. On the other hand, there were sections that were lacking significant explanation and contained so little detail that it was impossible to understand.

Also, if I had to read 'close proximity' one more time, I would have screamed. Someone should explain to the author AND THE EDITOR that this is redundant. I circled those words MULTIPLE times. Reading such a poorly written book made me wonder what else was poorly described and possible incorrect.

Principles and Practice of Modern Chromatographic Methods

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Principles and Practice of Modern Chromatographic Methods
Kevin Robards , P. R. Haddad , and P. E. Jackson
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0125895704

Book Description

Though many separation processes are available for use in todays analytical laboratory, chromatographic methods are the most widely used. The applications of chromatography have grown explosively in the last four decades, owing to the development of new techniques and to the expanding need of scientists for better methods of separating complex mixtures. With its comprehensive, unified approach, this book will greatly assist the novice in need of a reference to chromatographic techniques, as well as the specialist suddenly faced with the need to switch from one technique to another.

Size Exclusion Chromatography (Springer Laboratory)

Average customer rating:

Good book, but highly OVERPRICED

Size Exclusion Chromatography (Springer Laboratory)
Sadao Mori , and Howard G. Barth
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Handbook of Size Exclusion Chromatography and Related Techniques, Second Edition (Chromatographic Science)

Accessories:

ASIN: 3540656359

Book Description

Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Customer Reviews:

Good book, but highly OVERPRICED.......2001-08-30

This book introduces the fundamental concepts of Size Exclusion Chromatography with emphasis in the practical aspects, without going into many details, or mathematics, but gives the necessary information. It is very readable and it is not intended to the specialist in SEC, but for people who use it, especially in polymer analysis.
On the downside, it is very, very, very overpriced.

Average customer rating:

Short Protocols in Cell Biology

Manufacturer: Wiley
ProductGroup: Book
Binding: Paperback

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ASIN: 0471483397

Book Description

Providing condensed descriptions of more than 500 methods compiled from Current Protocols in Cell Biology, this text thoroughly explores cell biology in an easily accessible, hands-on format. Short Protocols in Cell Biology is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of cell biology methods.

Key Features:

Designed to provide quick access to step-by-step instructions for the essential methods used in every major area of cell biological research
Contains methods from every aspect of cell biologyeverything needed to study the basic structure and functions of cells at both the molecular and cellular levels

Laboratory Manual for the Examination of Water, Waste Water and Soil, 3rd Edition

Average customer rating:

Good Scientific Manual
A very good handbook

Laboratory Manual for the Examination of Water, Waste Water and Soil, 3rd Edition
Hans Hermann Rump
Manufacturer: Wiley-VCH
ProductGroup: Book
Binding: Paperback

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ASIN: 3527298258

Book Description

This successful laboratory manual is now available in its third edition. It has been thoroughly revised and updated. New features include:
- Methods for the determination of AOX, TOC and DOC
- Luminescent bacteria waste water test
- extended description of sample preparation for soil analysis
- comprehensive description of environmental protection measures in the laboratory
As in the previous editions, all methods for analysis described can be applied without consulting further literature. They have proven their practicability. Their description includes the necessary safety measures.
A review of the previous edition: 'One can recommend this book for people in professional training and students, but also for the practitioner, for whom this concise laboratory manual is a valuable aid for daily work.' (Fresenius Journal of Analytical Chemistry)

Customer Reviews:

Good Scientific Manual.......2005-01-19

Laboratory Manual for the Examination of Water, Waste Water, and Soil is a very informative. It starts out speaking about laboratory safety because this book talks about using chemicals and other substances that can be quite harmful. This was definitely one of the finer points of the book starting first and foremost with ways to avoid injury in the laboratory. Next the book goes into explaining how imperative quality control of the results of your laboratory work is. It explains that nothing is more important that the purity and accuracy of your results. The manual then goes into how to organize and catalogue your samples as to not get them confused with one another. In the next section the manual talks about how to take field measurements and how to properly and accurately record your results. It covers the entire test from ph, to calcium carbonate aggression. The last 2 sections go into how to take more detailed and accurate measurements back in the lab to the interpretation of all of the results that you come up with.
While this book uses great detail in explaining the subject on what it was written, as for any ability to hold my interest is where this was lacking. This manual was very dry it obviously was not written to be interesting or anything of that nature, But in all seriousness this was possibly one of the most boring books I have written. The language that was used in the manual was very technical and if you do not know the terminology then it is easy to get confused and lost. Plus this language adds to the boredom because of the frustration that comes with trying to understand the highly scientific terms. But for the purpose that this novel was written for it is perfectly written using precise detail and covering all of the bases and answering all of the questions that may arise in the course of testing water samples. I recommend this book for those who are interested in entering the field of water examination it is a excellent book for those who are looking to get there feet wet in this field. But if you are in search of a page-turner something to hold your attention or just a good read I suggest that you steer far away from this book it is not one that would hold any ones attention for any long stretch of time.

A very good handbook.......2000-10-27

This manual offers a very good basis not only for experts of water chemistry, water and waste water treatment and environmental protection but also for teachers and students of technical directions at colleges as well as at universities.

225 pages contain all factors playing an important role for examination of water, waste water and soil. 9 chapters include general remarks to laboratory safety (also regarding to waste treatment) and quality control. Furthermore requirements for analytical methods of the different water types and soil are mentioned. Further chapters deal with the preparation and technique of the sampling as well as their application in practice.

Chapter "Laboratory Measurements" describes all important methods in detail. So this book is a perfect handbook for the target group, even without using the attached extensive reference list.

In chapter 7 a huge variety of tables containing especially German legal and technical regulations is an clearly help for interpretation of received results.

This book can be highly recommended as a handbook for everybody dealing with water, waste water and soil treatment.

R. Bäßler

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