Discovering Genomics, Proteomics, and Bioinformatics

Book Description

Discovering Genomics, Proteomics, and Bioinformatics combines integrated Web exercises with a problem-solving approach to train readers in basic hands-on genomic analysis. The authors present global problems, then provide the tools of genomic analysis to help readers dissect the answer, thus encouraging critical thinking skills. Short boxed readings called "Math Minutes" explain the math behind the biology. KEY TOPICS: GENOMIC SEQUENCES, GENOMIC EXPRESSION, WHOLE GENOME PERSPECTIVE, TRANSITION FROM GENETICS TO GENOMICS: MEDICAL CASE STUDIES. For anyone interested in genomics, proteomics, or bioinformatics.

Customer Reviews:

Textbook + website = great new textbook.......2007-04-24

This was a great textbook. The website was very helpful and I liked how the author did not waste paper/printing/money on images and half of the information was on the web. It was nice not carrying around a heavy text all semester, even if half my reading/work was done in front of a computer. A lot of information packed into this book. One sentence sometimes requires a lot of knowledge (thank goodness for my professor who explained it all). I would have to say, without a lecture, I would walk away from this text thinking I knew something but not knowing much at all. For an amature like me, I definitely need a lecture to go along with this text. Although some mistakes were found, a lot less than the first edition (so I'm told).

Great New Format to get students out of a dull book.......2006-03-17

This book represents a breakthrough in textbook design. It starts with a 'case study' for a child visiting you the physician. You get the basic symptoms from the mother, then you are sent to the web to go attempt to establish a diagnosis. And you are not sent to some private web site, but to the Online Mendelian Inheitance in Man (OMIN) database of human diseases and genes, and to the National Center for Biotechnology Information. Immediately the student is exposed to a wealth of information far beyond what any book could provide. It's rare that you see a textbook that attempts to take the student into the real world.

Intermixed with the case studies is textual materials that provide the student with the basic background that they need. In addition there are almost random Math Minutes and Discovery Questions that direct the student into further depth of understanding.

If you are planning to teach this kind of class, you owe it to yourself to at least investigate this book before selecting a text.

Simply fascinating.......2004-12-08

The sheer number of books in the area of bioinformatics is growing rapidly, and each author takes a different approach to the topic: Bergeron's Bioinformatics Computing uses concepts from Information Theory, while Pervzner's Computational Molecular Biology uses Graph Theory and Durbin et. al. Biological Sequence Analysis use Statistics and hard core mathematical analysis to get the point across. Campbell and Heyer, the authors of Discovering Genomics, Proteomics, & Bioinformatics use concepts from Electrical Engineering and Control Engineering to prescribe some of the details of genomics and proteomics. As an instructor you need to choose the right book to meet the needs and understanding level of your target audience. For biology students that have little mathematics or statistics knowledge but have taken Physics II (E&M), this would be the perfect book. The authors are a little heaving on the biological terminologies, so a sophomore level Computer Science major with little or no biology background would have a difficult time coming up to speed at the beginning, but the glossary of terms at the end of the book is comprehensive enough that can aid the learning process.

The approach to figuring out what, where and how genes are controlled using what are called circuits is simply fascinating. Genes are controlled in three ways: location of the gene, the time of control and the amount. These three metrics can easily be mapped to circuit diagram in Electrical Engineering using the concept of switches (transistors) and time varying influencers (control theory and feedback loops), the authors describe of genes are regulated. One can imagine that the process of mapping genes into circuits is a difficult task, and one would be right. In fact, only one gene, the Endo16, has been fully expressed in circuit diagrams and that task took years of research. Imagine the amount of man power and hours it would take to draw the circuit diagram for the entire human genome of over 35000 genes! Even the concepts of noise in the circuit and complex integrated circuits are explored in details. The authors make no hesitation in telling the readers what the unknowns are and what topics are under intense investigation. It is amazing to realize that we still a long way to go, and what we are seeing thus far is just the beginning on a very long journey.

In general, the book is very interactive filled with Discovery Questions, Math Minutes and a CD simply filled with tons and tons of pictures that are in PDF format that can be printed in need be. There is also a Web site that accompanies this book that explains various bioinformatics methods, and contains information required by some of the Discovery Questions. The text itself is divided into four units:
1) Genomic Sequences which talks about the whole picture of genomic analysis and its benefits. The question of why this field of study is so important and what we hope to gain from in the coming years and decades is also answered. The students are exposed to topics in the areas of biological weapons, antibiotics and how they work, the evolution and survival of DNA and ethical consequences of genetics and genetic engineering.
2) Genomic Expressions in which the how and where questions are answered in detail. How did we gather all of this information? DNA microarrays are discussed in detail including advanced research in the area and who is doing in. Proteins microarrays are also discussed, but not in as much details due to the fact that protein microarrays are very much in their infancies. The authors do make a number of predictions in terms of where the proteomics area of research is heading and what we need to get there.
3) Whole Genome Perspective. The bottom up approach takes the readers to the whole genome analysis, and that's where the authors discuss the notions of electronic circuits in describing the genome and proteins prescription.
4) Medical Case studies in the areas of drug discovery bring this book to an end. The authors tackle questions such as how a disease is discovered and researched, and go into some of the misconceptions of drug discovery and end the book with why discovering a new drug is such a difficult task.

A complete text with the entire information one would need to come up to speed with the area of life sciences. A good mix of biology, mathematics, statistics, engineering and computer science give the reader a comprehensive overview of the current problems, research areas and new developments in the field of bioinformatics.

I recommend this text for a bioinformatics course with students of biology with little mathematics and statistics background. The text is easy to read and follow. The accompanying CD-Rom is field with relevant pictures, graphs, etc, that can further the learning process.

A novel approach!.......2003-03-22

Abstract: great and innovative book. I have seen many books, but none like this. It is still concise in this first edition, yet could become the "Lewin" of genomics.
Score: 9/10.

Recommended to students: yes, together with classic works like Brown.

Recommended to Central Library: yes.

1. The supplied CD-ROM is a nice teaching aid. Yet, it is difficult to "extract" pictures from it for teaching purposes. It would be much more useful if the pictures were individually supplied in standard high-quality graphic formats like TIFF, instead of PDF. The later is perfect for distributing text with pictures, but not to retrieve such pictures. Other publishers distribute the book artwork as individual TIFF files. That approach greatly enhances the book and boost sales. This is particularly useful for teachers. Actually, it is a must for us these days. Please, make sure that future versions of the CD-ROM or DVD-ROM are --as this one-- compatible with the open-source Unix-based Mac OS X platform. Thanks.

2. The associated web page "Instructor's Guide" 3. The discovering questions are terrific. Please, expand them in future versions.

4. Math minutes are an excellent idea.

5. Boxes are welcome. Please, include more.

6. Also helpful are the boldface words on each chapter. Perhaps they could be also included in a keywords at the beginning of each chapter.

7. The index should be more comprehensive and should have all main entries in boldface. This is important to any index and very few books have it right.

8. The glossary is helpful. It should be more comprehensive,
including more terms.

9. The summaries and conclusions are great, yet should be expanded to include more relevant information. They should be like a "minichapter" an the end of each chapter or --better-- at the beginning. All partial summaries could be pooled into a larger summary that way.

10. Addendum sections could be included as separate notes or boxes.

11. The pronunciation tips for new words are also an excellent idea; mostly for non-English speakers.

12. The classified references are really useful. Well done. If they were commented or "annotated" they would be just perfect.

13. A list of abbreviations would be welcome. A list sorted by the full name would be very handy as well.

14. What about telomerase and aging? What about the fact that
unicellular organisms are immortal? Or stem cells? Or tumor cells? Death is a tax that multicellular organisms have to pay to nature in order to evolve. Yet we humans might change that soon.

15. It should be clearly indicated the organisms with genomes made of dsDNA, ssDNA, dsRNA and ssRNA.

16. Missing bioinformatics tools and step-by-step analysis of genes and mRNA (see next) and whole genomes.

17. It would be really helpful to explain clearly and analyze --even from a bioinformatics point of view-- the structure of genes, mRNA, CDS, introns, exons, promoters and terminators. It is not clear where do these elements start or end or how to recognize them. Diagrams and graphs would greatly help to explain these absolutely basic and fundamental concepts. In other words, imagine that you have cloned and sequenced a genomic gene as well as a full mRNA (cDNA). Now you want to publish your results and for that you do a comprehensive description of your gene (chromosome) and cDNA (mRNA). That is precisely the kind of information that is missing as a diagram and explanation. In this way, it should be indicated that you may encounter several ATG (or other) starting coding triplets in the mRNA, that if the 20 or so amino acid residues of the 5'-end of a peptide have a high percentage of hydrophobic residues, they are likely part of a leading peptide which would be further excised, that you may encounter several polyadenylation signals, etc. On the genome side, the promoter and terminator structures should be analyzed, as well as the intron-exon boundaries.

18. Likewise, it should be indicated the tools and current
possibilities to determine or predict the 3D structure of a protein (folding) from the primary structure of the peptide.

19. Does not mention Lasergene package of DNAStar 20. Which genes are best to draw dendrograms? Differentiation between genes from the nucleus or organelles (mitochondrion or chloroplast). Likewise for DNA fingerprinting and molecular markers.

21. Differential display methodologies are missing (as well as other methodologies of gene expression like subtractive hybridization).

22. Large-scale sequencing is missing. For instance, sequencing of single-molecules will allow the sequencing of whole chromosomes or genomes.

23. Missing tables comparing different genomes with full details
(size, ploidy, percentage of genes, introns, exons, repetitive DNA, junk DNA, etc).

24. Reference to manufacturers is very useful. Please, include also links to web sites. Best if all manufacturers are included as an appendix.

25. All web sites (NCBI, etc) and web-based applications (BLAST, ORF Finder, etc) should be clearly indicted in an appendix.

26. It is not indicated that the PCR was in fact described with full details by Khorana et al 14 years before Mullis et al.

27. Please, include more drawings and pictures in the printed book and CD-ROM.

28. Suggestion: including chapters on eukaryotic-genomic DNA
libraries, cDNA libraries, subtractive libraries.

29. Suggestion: including chapters on plant and animal transformation.

30. Suggestion: including drawing of Maxam-Gilbert sequencing method and Sanger method (Applied Biosystems electropherograms,
electrofluorograms).

31. Prions, viroids and viruses could be also included.

32. A title index at the beginning of each chapter would be very
useful. Besides the goals for chapter, which are quite useful.

33. Bioinformatics could be significantly expanded.

34. QuickTime videos explaining some topics would be fantastic.
Please, make them in QuickTime (best quality, platform-independent).

Book Description

The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.
* Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
* Explains the hows and whys of using mass spectrometry in proteomic analysis
* Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
* Comments on future challenges and how they may be addressed
* Includes sections on troubleshooting

Download Description

"The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.

Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
Explains the hows and whys of using mass spectrometry in proteomic analysis
Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
Comments on future challenges and how they may be addressed
Includes sections on troubleshooting

Proteomics in Practice: A Laboratory Manual of Proteome Analysis

Average customer rating:

Proteomics in Practice: A Laboratory Manual of Proteome Analysis
Reiner Westermeier , and Tom Naven
Manufacturer: Wiley-VCH
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Analytic | Chemistry | Science | Subjects | Books

General | Science | Subjects | Books

Biochemistry | Basic Science | Medicine | Subjects | Books

German | Foreign Language Nonfiction | Nonfiction | Subjects | Books

Look Inside Nonfiction Books | Trip | Specialty Stores | Books

Look Inside Science Books | Trip | Specialty Stores | Books

Nonfiction | German | Foreign Language Books | Specialty Stores | Books

Professional & Technical | German | Foreign Language Books | Specialty Stores | Books

All German Books | German | Foreign Language Books | Specialty Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Nonfiction | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 3527303545

Book Description

A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries.
The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail.
Intended for all those wishing to go beyond the theoretical aspects of proteome analysis, this book is targeted at research groups within academia and industry, course instructors, research assistants and graduate students.
Special feature: Updates of recipes as well as animations illustrating crucial methodological steps are available on the Web at http://www.wiley-vch.de/books/info/3-527-30354-5

Proteins and Proteomics: A Laboratory Manual

Average customer rating:

An OK book but reinvents the wheel
The "must have" book for the practicing proteomics lab .

Proteins and Proteomics: A Laboratory Manual

Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Paperback

Biochemistry | Biological Sciences | Science | Subjects | Books

General | Science | Subjects | Books

General | Reference | Science | Subjects | Books

Biochemistry | Basic Science | Medicine | Subjects | Books

Look Inside Science Books | Trip | Specialty Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 0879695544

Book Description

Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.

Customer Reviews:

An OK book but reinvents the wheel.......2004-06-16

To be fair this is a good and useful book, but at a higher price than the already excellent published (2002) laboratory proteomics by Westermeier and Naven, save your dollars. The latter goes for half the price and serves the same purpose with excellent illustrations and protocols.

The "must have" book for the practicing proteomics lab ........2004-04-19

This is a outstanding book, covering all aspects of sample preparation and experimental techniques involved in protein and peptide analysis by gels, liquid chromatography and mass spectrometry. Each chapter gives detailed protocols complete with tips, reagent lists, short tutorials (printed in blue colored information panels) and references. The protocols will work for you just as they are written and will give you a place to start in customizing and adapting them to your own circumstances (and you certainly will do that!). As with any fast moving field an updated second edition will be welcome around 2006, but by then, I'll be happy to replace my dog-eared copy for a fresh one (or maybe not, I don't really think a book as good as this will ever be obsolete). I recommend you buy the paperback edition, it will serve you well and save you some $$$.

Principles of Proteomics (Advanced Text Series)

Average customer rating:

A moderately good source for someone pursuing proteomics
A very good introduction

Principles of Proteomics (Advanced Text Series)
R. M. Twyman
Manufacturer: BIOS Scientific Publ
ProductGroup: Book
Binding: Paperback

Biochemistry | Biological Sciences | Science | Subjects | Books

Biotechnology | Biological Sciences | Science | Subjects | Books

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

General | Engineering | Professional & Technical | Subjects | Books

Biotechnology | Special Topics | Medicine | Subjects | Books

Look Inside Science Books | Trip | Specialty Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 1859962734

Book Description

The Advanced Text series is designed for students taking advanced-level courses, including upper-level undergraduates and graduate students. Titles in this series will also be invaluable to researchers new to a field, and to established researchers as a basic reference text.

Proteomics is a major new area of scientific research driven by the growing information gap between genes and their functions. Large-scale DNA sequencing and annotation projects identify thousands of new genes on a daily basis but the functions of these genes remain largely unknown. Proteins are the actual functional molecules of the cell, and technical advances in the high-throughout analysis of proteins now allow these molecules to be studied directly. Principles of Proteomics provides a comprehensive introduction to this relatively new field, for the first time bringing together all the disparate themes of proteomics. The book covers the development of large-scale technologies for protein separation, isolation,detection and quantitation. It describes in detail the role of mass spectrometry in high-throughput protein annotation. It also covers the emerging fields of structural proteomics, interaction proteomics and the recent development of chip-based analysis techniques. The final chapter provides some examples of how proteomics is being applied in the fields of medicine, biotechnology and agriculture. Each chapter ends with a short bibliography containing some classic papers and useful reviews for further reference.

Customer Reviews:

A moderately good source for someone pursuing proteomics.......2007-06-28

The illustrations were the best I have seen for a text explaining this subject. There are not alot of books about proteomics out there, but this is a must have for the introduction to this wonderful yet esoteric field. If you have no previous knowledge of biochemistry this book will be a very tough read. Enjoy.

A very good introduction.......2006-02-19

This is a very good introduction and general review of the field of proteomics. It is excellent for those with little or no experience in the field. Because of the rapid changes occurring in this new science it does not include review of many of the useful software tools that have been developed.

Average customer rating:

Captures the State of the Art and Points to New Potentials

Informatics In Proteomics

Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Biotechnology | Biological Sciences | Science | Subjects | Books

Bioinformatics | Biological Sciences | Science | Subjects | Books

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

Prosthesis | Special Topics | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1574444808

Book Description

The handling and analysis of data generated by proteomics investigations represent a challenge for computer scientists, biostatisticians, and biologists to develop tools for storing, retrieving, visualizing, and analyzing genomic data. Informatics in Proteomics examines the ongoing advances in the application of bioinformatics to proteomics research and analysis. Through computer simulations, scientists can determine more about how diseases affect cells, predict how various drug interventions would work, and ultimately use proteins as therapeutic targets. This book first addresses the infrastructure needed for public protein databases. It discusses information management systems and user interfaces for storage, retrieval, and visualization of the data as well as issues surrounding data standardization and integration of protein sequences recorded in the last two decades. The authors subsequently examine the application of statistical and bioinformatic tools to data analysis, data presentation, and data mining. They discuss the implementation of algorithms, statistical methods, and computer applications that facilitate pattern recognition and biomarker discovery by integrating data from multiple sources. This book offers a well-rounded resource of informatic approaches to data storage, retrieval, and protein analysis as well as application-specific bioinformatic tools that can be used in disease detection, diagnosis, and treatment. Informatics in Proteomics captures the current state-of-the-art and provides a valuable foundation for future directions.

Customer Reviews:

Captures the State of the Art and Points to New Potentials.......2005-08-19

Once considered a pretty well known part of biology, the study of proteins has become one of the most exciting, fastest changing area. The advent of molecular-based methods of genomics combined with new techniques in bioinformatics has opened dramatic new areas for research that offer some promise in the treatment of cancer.

Such a rapidly changing field is difficult to capture in a book written by any individual, it's simply changing too rapidly. In thisbrand new book (published June 28, 2005) Dr. Srivastava has gotten many of the most advanced researchers in the field to contribute articles that truly define the state of the art as it exists today. It also provides a vision for where the field is heading, including several chapters that point into areas where new research may yield significant results.

New and Emerging Proteomic Techniques (Methods in Molecular Biology)

Average customer rating:

New and Emerging Proteomic Techniques (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Biotechnology | Biological Sciences | Science | Subjects | Books

Anatomy | Biological Sciences | Science | Subjects | Books

General | Science | Subjects | Books

Biochemistry | Basic Science | Medicine | Subjects | Books

General | Pharmacology | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1588295192

Book Description

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Purifying Proteins for Proteomics: A Laboratory Manual

Average customer rating:

Purifying Proteins for Proteomics: A Laboratory Manual

Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Paperback

Biochemistry | Biological Sciences | Science | Subjects | Books

General | Science | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 0879696966

Book Description

This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The protocols are accompanied by extensive discussion of potential pitfalls and troubleshooting advice.

Introduction to Proteomics: Tools for the New Biology

Average customer rating:

Very readable introduction to Proteomics
Good information, poor organization

Introduction to Proteomics: Tools for the New Biology
Daniel C. Liebler
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Research | Education | Science | Subjects | Books

Methodology & Statistics | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Biochemistry | Basic Science | Medicine | Subjects | Books

General | Pharmacology | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 0896039919

Book Description

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Customer Reviews:

Very readable introduction to Proteomics.......2004-02-01

This very readable book provides an excellent overview of proteomics. It gives a survey of techniques, comparing them and explaining why and when they are used. This is not the place to learn any method in detail. But it is the only book I have seen that you can read cover to cover in a few days and get the big picture of the field. Some caveats: each chapter has only 3 or 4 references, and the book focuses on methods connected to mass spectrometry. But if you want a quick and pleasant introduction to methods for purifying and identifying proteins, including discussions of ICAT, protein complexes and post-translation modifications, I highly recommend this book.

Good information, poor organization.......2003-11-04

There really are lots of good facts in this book. Somehow, though, they never come together to form a coherent whole.

Most chapters read like essays, meant to stand on their own. That means that many chapters repeat introductory material already covered, and remake points already made. Yes, I appreciate that silver staining does not properly represent the whole range of protein expression levels. I do not appreciate the need to stat that three or four times.

Despite its "Introduction" title, the author seems to forget what a novice will need to learn. For example, there's a discussion of isoelectric focussing for protein separation. That includes mention of the technique's history, and why it used to have reproducibility problems. I never did see an exact description of what the techique actually is or how it works, though. Likewise, the author describes a number of important software packages used in proteomics. There are no screen shots, though, comparisons of capabilities, details of usage, or even references for letting me find out more on my own.

When I got to the the end of this book, I wanted to go back and review a few points. Because of the generally poor organization, it was always a struggle to figure out just which discussion covered the topics I wanted.

Book Description

Hubert Rehm's Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides the reader with tips and tricks for more successful lab experiments. Using a conversational yet professional tone, Rehm provides an overview of a variety of methods in protein biochemistry/proteomics. He provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. Readers will immediately sense that the author if very familiar with the challenges, and frustration of the daily lab routine. Never before has such an honest, tactical guide been available for those conducting lab experiments within the field of biochemistry.

· Shows how to avoid experimental dead ends and helps users develop an instinct for the right experiment at the right time
· Contains short and precise summaries of routine procedures (e.g. column chromatography, gel electrophoresis), and lists the advantages and disadvantages of alternative methods
· Includes over 100 detailed figures and tables
· Contains a chapter on proteomics

Customer Reviews:

good book - misprinted edition - missing / blank pages in Ch.2.......2007-07-30

In the chapter 2, a number of important pages (50-51, 54-55, 58-59, 62-63, 66-67, 70-71, 74-75, 78-79) is missed / left blank - apparently by the publisher's fault! This is really frustrating - a good book is ruined by a printing mishap!

really helpful.......2006-10-31

I've found this book really very helpful. I'm essentially a geneticist/molecular biologist, but with this book I've been able to develop a multistep protocol for affinity purifying my protein of interest. The book is clear and concise, I would highly recommend it.

Books:

Books Index

Books Home

Recommended Books