Basic HPLC and CE of Biomolecules

Book Description

This text provides explanations and examples of the primary chromatographic techniques for biomolecules (reversed-phase, size exclusion, ion-exchange, hydrophobic interaction and affinity). It also offers practical suggestions for their implementation and optimization. An extensive bibliography furnishes a wealth of background information.

Customer Reviews:

So brilliant that I cried--twice!.......2003-04-25

This is without a doubt the most brilliant and poignant depiction of HPLC and CE of biomolecules in modern history. My extreme hyperactivity disorder usually means that I'm not able to sit still for such weighty topics--not without large amounts of abused Ritalin; but Cunico's work was so compelling and finely wrought that I found myself spellbound, even when drunk, even when driving in the backseat of a stolen car across the Mexican border at Tecate. I am not going to recommend drinking heavily and stealing cars. But I do recommend BASIC HPLC and CE OF BIOMOLECULES by Robert L. Cunico. By the way, I am extremely drunk right now.

Basic HPLC and CE of Biomolecules.......2000-12-24

This is a book that should be on the desk of anyone who is involved in analytical development of biomolecules and biopharmaceuticals. The content is well organized and written in a format that is easy to grasp and understand. The content covers almost all the different modalities of HPLC analyses and applications and provides real life examples of the techniques. It includes innumerable suggestions on how best to develop a validatable HPLC method and analyze the data appropriately.

Book Description

Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines.

Capillary Electrophoresis Methods for Pharmaceutical Analysis

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Capillary Electrophoresis Methods for Pharmaceutical Analysis
George Lunn
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471331880

Book Description

Capillary Electrophoresis Techniques for Pharmaceutical Analysis at your fingertips-A complete, up-to-date library.
This invaluable database--also available on CD-ROM--gives you fast, easy access to the growing literature on capillary electrophoresis (CE). Nearly 3,000 abstracts from approximately 900 publications make up this comprehensive library, listing CE techniques for the analysis of more than 700 active pharmaceutical compounds. From acarbose to mephobarbital to zopiclone--a number of procedures are grouped together for each compound. Detailed, precise information lets you replicate methods without referring to original articles as well as customize methods for specific needs. Features include:
* Methods for drugs in biological fluids such as blood or urine as well as for bulk and formulated drugs
* Name and structure of each target compound as well as molecular weight and formula, CAS registry numbers, and Merck index number
* Experimental conditions for each method, including sample preparation, analytical matrix, capillary/capillary instrument model, capillary temperature, interfering compounds, and more
* Bibliography of reviews of capillary electrophoresis procedures
The CD-ROM additionally combines the four-volume HPLC Methods for Pharmaceutical Analysis with thousands of methods listed for more than 1,300 compounds. This immensely useful reference will save you countless hours of online and library research.

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Finding the Middle Ground
Useful text
Waste of money
Badly written

Practical Capillary Electrophoresis
Robert Weinberger
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0127423567

Book Description

In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry.

Key Features
* Illustrated with detailed diagrams of electrophoretic phenomena
* Offers step-by-step methods development schemes
* Presents techniques for developing quantitative, robust, and precise methods
* Includes an extensive troubleshooting guide
* Updates and greatly expands on the first edition-more than 50% of the text is new
* Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Customer Reviews:

Finding the Middle Ground.......2003-01-26

This text finds the middle ground between the theory and practice of capillary electrophoresis. The text is ideal for students as well as industrial practitioners in the field

Useful text.......2002-01-18

This a very useful text for students and experienced users alike. The book is the right level of information regarding instrumentation, basic concepts and provides useful information on various applications of the technology. Definitely a must buy for the novice and student -- likely a useful reference text for the experienced user.

Waste of money.......2001-09-11

There are half a dozen better books on the subject.

Badly written.......2001-08-08

The confusing presentation, clumsy writing, and numerous factual errors make this book almost unreadable.

Analysis and Detection by Capillary Electrophoresis, Volume XLV (Comprehensive Analytical Chemistry) (Comprehensive Analytical Chemistry)

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Analysis and Detection by Capillary Electrophoresis, Volume XLV (Comprehensive Analytical Chemistry) (Comprehensive Analytical Chemistry)

Manufacturer: Elsevier Science
ProductGroup: Book
Binding: Hardcover

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ASIN: 0444517189
Release Date: 2005-07-01

Book Description

Capillary Electrophoresis (CE) is a powerful analytical technique used to separate compounds and is increasingly being used in routine analytical laboratories. Analysis and Detection by Capillary Electrophoresis presents developments enabling the enhancement of the detection sensitivity in CE, including the different strategies used to achieve sensitivity requirements. It describes techniques allowing sample preconcentration and sensitive continuous detection systems and looks at recent developments such as chiral analysis in CE and electrochemical detection in microchips. UV-Vis absorbance detection, as the most widely used detection system in CE, is also presented. Analysis and Detection by Capillary Electrophoresis delves into the practical approaches used in the field and will greatly benefit analytical chemists, as well as students, teachers, technical analysts, scientists and researchers involved in capillary electrophoresis.

* An overview of practical approaches employed in capillary electrophoresis, including the fundamentals, instrumentation, and applications of continuous detections systems employed in capillary electrophoresis
* Covers aspects of capillary electrophoresis that have never previously been covered
* Written by leading experts in the field

Capillary Electrophoresis Guidebook: Principles, Operation, and Applications (Methods in Molecular Biology)

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Capillary Electrophoresis Guidebook: Principles, Operation, and Applications (Methods in Molecular Biology)
Kevin D. Altria
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896033155

Book Description

Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties.

The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.

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Capillary Electrophoresis
S. F. Y. Li
Manufacturer: Elsevier Science Pub Co
ProductGroup: Book
Binding: Hardcover

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Book Description

Hardbound. Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis.

Book Description

John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Pharmaceutical & Biomedical Applications of Capillary Electrophoresis (Progress in Pharmaceutical and Biomedical Analysis)

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Pharmaceutical & Biomedical Applications of Capillary Electrophoresis (Progress in Pharmaceutical and Biomedical Analysis)
S.M. Lunte , and D.M. Radzik
Manufacturer: Pergamon
ProductGroup: Book
Binding: Hardcover

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Book Description

The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis.

Clinical Applications of Capillary Electrophoresis (Methods in Molecular Medicine)

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Clinical Applications of Capillary Electrophoresis (Methods in Molecular Medicine)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Book Description

Stephen M. Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including serum and urine proteins, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, and cathepsin D. The protocols offered for DNA analysis include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microstaellite instability in cancers. Many of the methods can be automated to replace more labor intensive and costly tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods.

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