Practical HPLC Method Development, 2nd Edition

Book Description

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Customer Reviews:

A great book - information overload.......2002-07-16

This is a very useful reference to have if you work extensively on HPLC. I find its of more use to understand concepts ...there is a lot of information here.

Which means - if you are looking for a solution to a problem and you want to research separation theory - a great book.

If your looking for a quick fix to an HPLC problem - not as good. This is a detailed, extensive and well written text on HPLC Method development - wish there was more on validation here though.

Plactical and Rational method Development!.......2000-03-15

Rational Method development for HPLC is resented. Instruction of rational development of 3-solvent separation system is excellent. This book also has useful know-hows and tools in practical laboratory experiments, such as strength of hydrogen bonding of base silica and nomograph for estimation of "solvent strength" for reversed phase HPLC.

an excellent book on pratical HPLC.......1998-01-20

The book is useful for gerenal operator who are both novice and expert. It do is pratice. It's better give another chapter for troubleshooting and maintence.

Average customer rating:

a very good start point

LC/MS: A Practical User's Guide
Marvin McMaster
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471655317

Book Description

A practical guide to using and maintaining an LC/MS system

The combination of liquid chromatography (LC) and mass spectrometry (MS) has become the laboratory tool of choice for a broad range of industries that require the separation, analysis, and purification of mixtures of organic compounds.

LC/MS: A Practical User's Guide provides LC/MS users with an easy-to-use, hands-on reference that focuses on the practical applications of LC/MS and introduces the equipment and techniques needed to use LC/MS successfully. Following a thorough explanation of the basic components and operation of the LC/MS system, the author presents empirical methods for optimizing the techniques, maintaining the instrumentation, and choosing the appropriate MS or LC/MS analyzer for any given problem.

LC/MS covers everything users need to know about:

The latest equipment, including quadrupole, time-of-flight, and ion trap analyzers
Cutting-edge processes, such as preparing HPLC mobile phases and samples; handling and maintaining a wide variety of silica, zirconium, and polymeric separation columns; interpreting and quantifying mass spectral data; and using MS interfaces
Current and future applications in the pharmaceutical and agrochemical industries, biotechnology, clinical research, environmental studies, and forensics

An accompanying PowerPoint® slide-set on CD-ROM provides vital teaching tools for instructors and new equipment operators. Abundantly illustrated and easily accessible, the text is designed to help students and practitioners acquire optimum proficiency in this powerful and rapidly advancing analytical application.

Download Description

Presents technical data in an easily understood format, explains how to keep LC/MS systems up and running, and shows how to interpret and quantitate the mass spectral data using proven spectral databases, without devolving into a university-style course curriculum. Takes a purely practical approach to using and maintaining LC/MS systems Introduces the reader to quadrupole and ion trap analyzers, which make up the bulk of the equipment being sold for laboratory and analytical studies Presents technical data in an illustrated, easily understood format Explains how to keep systems up and running Shows how to interpret and quantitate mass spectral data using proven spectral databases

Customer Reviews:

a very good start point.......2006-04-24

A good introduction. But not for specialist. Some practical tips are useful.

Troubleshooting HPLC Systems: A Bench Manual

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Troubleshooting HPLC Systems: A Bench Manual, by Sadek

Troubleshooting HPLC Systems: A Bench Manual
Paul C. Sadek
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Spiral-bound

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ASIN: 0471178349

Book Description

A complete, up-to-date guide to the use, maintenance, and troubleshooting of HPLC systems

The last twenty-five years have seen a dramatic rise in the use of High Performance Liquid Chromatography (HPLC) in laboratories worldwide. Troubleshooting HPLC Systems provides analysts as well as laboratory technicians and managers with a readily accessible and immensely useful guide to the new generation of HPLC equipment and techniques. With an emphasis on effective troubleshooting of HPLC systems, this lab companion covers system configuration and functions, problem-solving procedures, maintenance, and HPLC basics. It then walks chromatographers investigating the source of a malfunction through each system component-from solvents and reservoirs to sample preparation to columns and detectors. Special features of Troubleshooting HPLC Systems include:
* A detailed review of HPLC instrumentation and accessories
* The role of operating parameters as indicators of system performance
* Step-by-step troubleshooting protocols for each system component
* How to set up a preventive maintenance program for HPLC systems
* An overview of the categories of HPLC separations
* A compilation of HPLC terms and definitions
* Tables and charts detailing solvents' properties

Customer Reviews:

Troubleshooting HPLC Systems: A Bench Manual, by Sadek.......2001-09-30

I thought that this was an excellent book. It provided lots of the practical kind of details that I was looking for. This book is great for someone who has either limited or no experience in HPLC. It would also work as a refresher, for someone who has been out of the field for some time.

Average customer rating:

MOdern HPLC for Practicing Scientists
Excellent book
Excellent reference
A great reference/guide for HPLC practitioners
The Best Reference for Analytical Scientists

Modern HPLC for Practicing Scientists
Michael W. Dong
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Paperback

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ASIN: 047172789X

Book Description

A comprehesive yet concise guide to Modern HPLC

Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers.

Topics covered include:

HPLC operation
Method development
Maintenance and troubleshooting
Modern trends in HPLC such as quick-turnaround and "greener" methods
Regulatory aspects

While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources.

With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Customer Reviews:

MOdern HPLC for Practicing Scientists.......2007-06-04

HPLC has become one of the most widely applied chemical analytical techniques in the pharmaceutical industry. Dr Dong's book, Modern HPLC for Practicing Scientists, provides excellent source of information and knowledge for both beginners and highly experienced professionals who utilize this analytical tool. Chapter 1 presents an overview of chromatographic principles while the 2nd chapter deals with fundamental aspects of HPLC and considerations that enhance efficient separation. In chapter 3, Dr. Dong discusses the chromatographic column both from theoretical perspectives and applications. This is very valuable to practicing scientists.

In Chapter 4, the book provides a concise overview of HPLC instrumentation, operating principles as well as recent advances in instrumentation such as Waters Acquity UPLCTM (Chapter 11). A lot of information is provided on practical issues associated with the use of HPLC systems and the pictures and illustrations make their understanding much easier.

Chapter 5 provides a hands-on approach to HPLC analysis including mobile phase preparation and parameter optimization. This is a great chapter for a novel analyst who is often saddled with numerous options on the choice of HPLC parameters. Dr. Dong's attention to details makes it possible even for the novice chromatographer to undertake a full HPLC procedure with little or no supervision

Although Chapters 6 and 7 deal with applications in the pharmaceutical, food and environmental industries, the practicing scientist who this text is best suited for, would benefit more by integrating information from these chapters with the fundamental and practical concepts documented in the earlier chapters.

The inclusion of method development and regulatory issues with respect to HPLC techniques and applications in chapters 8 and 9 is particularly commendable. Industrial R & D scientists who are not only challenged to develop and optimize methods but also work within the constraints of regulatory agencies would find these chapters immensely rewarding. These chapters provide hands-on guidance for laboratory scientists in the pharmaceutical and other regulatory industries.

Chapter 10, dealing with troubleshooting is one of the special attributes of this book. This chapter provides quick fix approach to resolving some of the HPLC's often encountered problems. This chapter is bound to save some cost because more often than not, the HPLC analyst tend to vainly envisage and explore more catastrophic problems ignoring the simple remedies that are succinctly documented in Chapter 10.

Overall, Dr. Dong's book is very well written. Each aspect of the text is clearly and succinctly presented, making it easy to understand. The chapter on maintenance and troubleshooting guide (Chapter 11) should be a great asset. Dr. Dong has managed, in a very basic but meticulous manner, to produce an essential text for both the novice and experienced HPLC practitioner in the pharmaceutical industry.

Excellent book.......2007-05-10

This is one of the best books on HPLC I have encountered. Gives an extremely thorough introduction to everything you should know inorder to get started and work confidently with this indispensible technology. Invaluable for the graduate student who wants to go hands on.

Excellent reference.......2006-10-17

This is an excellent book that all liquid chromatographers should have. It covers all aspects of HPLC from theories to instrument to applications. It would benefit all levels of analytical scientists in their day-to-day operations. The book is informative and concise. It also includes an excellent list of references for further interest. I think Michael Dong has done it once again!!! Quite an impressive book! and the price can't be beat!

A great reference/guide for HPLC practitioners.......2006-10-02

This is quite a comprehensive treatise on the topic of HPLC. The intended audience is the novice/intermediate and it certainly hit the mark. It is quite apparent that the wealth of recommendations/pointers/guidelines comes from someone very familiar with this topic. This book should be of great help to many aspiring LC chromatographers and should also serve as a valuable reference to those that are already comfortable in this field. - Wilhad M Reuter (PerkinElmer LAS)

The Best Reference for Analytical Scientists.......2006-08-01

I have worked in the biotech industry (and the most well-known biotech company) for more than 10 years and found that this is by far the best HPLC reference book. I haven't seen any other book that contains more insightful information than Dr. Dong's.

This book is worth the price and my time of readig it.

Average customer rating:

HPLC for Pharmaceutical Scientists
Yuri V. Kazakevich , and Rosario LoBrutto
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471681628

Book Description

HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry.

In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided.

This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development.

Book Description

Stability-Indicating HPLC Methods for Drug Analysis compiles summaries of stability-indicating HPLC analytical methods that have appeared in the published literature. A first stop for pharmaceutical scientists, analytical chemists, and librarians in the quest for information about the stability of drugs. Co-published by the American Pharmaceutical Association and the Pharmaceutical Press, a division of the Royal Pharmaceutical Society of Great Britain.

Pulsed Electrochemical Detection in High-Performance Liquid Chromatography

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Pulsed Electrochemical Detection in High-Performance Liquid Chromatography
William R. LaCourse
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471119148

Book Description

A reliable, comprehensive, relevant view of HPLC and its applications

The development of HPLC-PED represents the successful marriage of two powerful analytical technologies and has resulted in the best technique for sensitive and direct detection of biological compounds with poor optical detection properties. PED has been used extensively for the determination of carbohydrates and other polar aliphatic compounds, and as a result, numerous methods have been developed to enable the analysis of a wide variety of samples. Over the years, many articles, anecdotal information, and misinformation have permeated the scientific community, with the possible consequence of confusion or uncertainty with regard to PED on the part of the analyst. Pulsed Electrochemical Detection in High-Performance Liquid Chromatography presents a reliable, comprehensive, and relevant review of HPLC-PED and its applications.

The book is divided into three major parts: background material necessary for a more thorough understanding of the principles and relevance of PED; an in-depth discussion of PED using voltammetry and other electroanalytical techniques and presenting the advantages, applicability, and optimization of all existing PED waveforms; and practical aspects of HPLC-PED, including a summary of the major applications and a look at future developments in the technique. Appendices include a pulsed voltammetry (PV) program specifically written to optimize pulsed amperometric detection (PAD) waveforms and all the known applications, categorized and listed in tabular form.

For analytical chemists; biochemists; carbohydrate chemists; biotechnologists; undergraduate, graduate, and postdoctoral students; and lab technicians working in a range of areas including the pharmaceutical, medical, and food and beverage industries, this eminently readable guide is the first reliable book-length treatment of how to use PED coupled with HPLC.

Average customer rating:

So brilliant that I cried--twice!
Basic HPLC and CE of Biomolecules

Basic HPLC and CE of Biomolecules
Robert L. Cunico , Karen M. Gooding , and Tim Wehr
Manufacturer: Bay Bioanalytical Laboratory, Inc.
ProductGroup: Book
Binding: Paperback

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ASIN: 0966322908

Book Description

This text provides explanations and examples of the primary chromatographic techniques for biomolecules (reversed-phase, size exclusion, ion-exchange, hydrophobic interaction and affinity). It also offers practical suggestions for their implementation and optimization. An extensive bibliography furnishes a wealth of background information.

Customer Reviews:

So brilliant that I cried--twice!.......2003-04-25

This is without a doubt the most brilliant and poignant depiction of HPLC and CE of biomolecules in modern history. My extreme hyperactivity disorder usually means that I'm not able to sit still for such weighty topics--not without large amounts of abused Ritalin; but Cunico's work was so compelling and finely wrought that I found myself spellbound, even when drunk, even when driving in the backseat of a stolen car across the Mexican border at Tecate. I am not going to recommend drinking heavily and stealing cars. But I do recommend BASIC HPLC and CE OF BIOMOLECULES by Robert L. Cunico. By the way, I am extremely drunk right now.

Basic HPLC and CE of Biomolecules.......2000-12-24

This is a book that should be on the desk of anyone who is involved in analytical development of biomolecules and biopharmaceuticals. The content is well organized and written in a format that is easy to grasp and understand. The content covers almost all the different modalities of HPLC analyses and applications and provides real life examples of the techniques. It includes innumerable suggestions on how best to develop a validatable HPLC method and analyze the data appropriately.

Book Description

An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include:
* Comprehensive overview of state-of-the-art HPLC column technology
* Explanation of the underlying principles of HPLC columns
* Methods for selecting columns
* Practical advice on using and applying columns, including examples
* Section by M. Zoubair El Fallah on methods development
* Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed
* Troubleshooting section
HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.

High-Performance Liquid Chromatography and Lipids: A Practical Guide

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High-Performance Liquid Chromatography and Lipids: A Practical Guide
William W. Christie
Manufacturer: Pergamon
ProductGroup: Book
Binding: Hardcover

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