Practical HPLC Method Development, 2nd Edition

Book Description

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Customer Reviews:

A great book - information overload.......2002-07-16

This is a very useful reference to have if you work extensively on HPLC. I find its of more use to understand concepts ...there is a lot of information here.

Which means - if you are looking for a solution to a problem and you want to research separation theory - a great book.

If your looking for a quick fix to an HPLC problem - not as good. This is a detailed, extensive and well written text on HPLC Method development - wish there was more on validation here though.

Plactical and Rational method Development!.......2000-03-15

Rational Method development for HPLC is resented. Instruction of rational development of 3-solvent separation system is excellent. This book also has useful know-hows and tools in practical laboratory experiments, such as strength of hydrogen bonding of base silica and nomograph for estimation of "solvent strength" for reversed phase HPLC.

an excellent book on pratical HPLC.......1998-01-20

The book is useful for gerenal operator who are both novice and expert. It do is pratice. It's better give another chapter for troubleshooting and maintence.

Average customer rating:

a very good start point

LC/MS: A Practical User's Guide
Marvin McMaster
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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GC/MS: A Practical User's Guide

ASIN: 0471655317

Book Description

A practical guide to using and maintaining an LC/MS system

The combination of liquid chromatography (LC) and mass spectrometry (MS) has become the laboratory tool of choice for a broad range of industries that require the separation, analysis, and purification of mixtures of organic compounds.

LC/MS: A Practical User's Guide provides LC/MS users with an easy-to-use, hands-on reference that focuses on the practical applications of LC/MS and introduces the equipment and techniques needed to use LC/MS successfully. Following a thorough explanation of the basic components and operation of the LC/MS system, the author presents empirical methods for optimizing the techniques, maintaining the instrumentation, and choosing the appropriate MS or LC/MS analyzer for any given problem.

LC/MS covers everything users need to know about:

The latest equipment, including quadrupole, time-of-flight, and ion trap analyzers
Cutting-edge processes, such as preparing HPLC mobile phases and samples; handling and maintaining a wide variety of silica, zirconium, and polymeric separation columns; interpreting and quantifying mass spectral data; and using MS interfaces
Current and future applications in the pharmaceutical and agrochemical industries, biotechnology, clinical research, environmental studies, and forensics

An accompanying PowerPoint® slide-set on CD-ROM provides vital teaching tools for instructors and new equipment operators. Abundantly illustrated and easily accessible, the text is designed to help students and practitioners acquire optimum proficiency in this powerful and rapidly advancing analytical application.

Download Description

Presents technical data in an easily understood format, explains how to keep LC/MS systems up and running, and shows how to interpret and quantitate the mass spectral data using proven spectral databases, without devolving into a university-style course curriculum. Takes a purely practical approach to using and maintaining LC/MS systems Introduces the reader to quadrupole and ion trap analyzers, which make up the bulk of the equipment being sold for laboratory and analytical studies Presents technical data in an illustrated, easily understood format Explains how to keep systems up and running Shows how to interpret and quantitate mass spectral data using proven spectral databases

Customer Reviews:

a very good start point.......2006-04-24

A good introduction. But not for specialist. Some practical tips are useful.

High-Performance Liquid Chromatography and Lipids: A Practical Guide

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High-Performance Liquid Chromatography and Lipids: A Practical Guide
William W. Christie
Manufacturer: Pergamon
ProductGroup: Book
Binding: Hardcover

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Practical Hplc Method Development Chromatography Method Development

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Practical Hplc Method Development Chromatography Method Development
Lloyd R. Snyder , Joseph L. Glajch , and Joseph J. Kirkland
Manufacturer: John Wiley & Sons Inc
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Binding: Hardcover

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Average customer rating:

Great intro to HPLC
Useful text for beginning HPLC user

HPLC: A Practical User's Guide
Marvin McMaster
Manufacturer: Wiley
ProductGroup: Book
Binding: Hardcover

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Modern HPLC for Practicing Scientists
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Practical HPLC Method Development, 2nd Edition

ASIN: 0471754013

Book Description

This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete with a CD-ROM and appendices, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds.

Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.

Customer Reviews:

Great intro to HPLC.......2003-09-05

I have recently been put in charge of an HPLC while having little experience in the field. This book was invaluable in developing my technique and was a gem as far as trouble-shooting goes (which I needed quite badly, I made a lot of mistakes at first). The only downside is that there hasn't been a new edition in almost 10 years, DAD and some of the processing have changed quite a lot and I would like to see that included if a future edition is made.

Useful text for beginning HPLC user.......1998-09-11

This was the most useful and straightforward text I came across in my search for HPLC information. As a beginning user, I found the sections on troubleshooting, column aging diagnosis and healing, and method development to be invaluable in getting an HPLC system up and running in a new laboratory. I would highly recommend this reference tool to beginning users who do not have the opportunity to interact with an experienced HPLC user. One consideration when choosing this book is that it does not go into detail on any theory behind method development; however, it does offer sound advice on developing methods.

High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model

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High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model
Lloyd R. Snyder , and John W. Dolan
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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Practical HPLC Method Development, 2nd Edition

ASIN: 0471706469

Book Description

Gradient elution demystified

Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed.

Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool.

This up-to-date, practical, and comprehensive treatment of gradient elution:
* Provides specific, step-by-step recommendations for developing a gradient separation for any sample
* Describes the best approach for troubleshooting problems with gradient methods
* Guides the reader on the equipment used for gradient elution
* Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients
* Explains how to avoid problems in transferring gradient methods

With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

A Practical Handbook of Preparative HPLC

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A Practical Handbook of Preparative HPLC
Donald A Wellings
Manufacturer: Elsevier Science
ProductGroup: Book
Binding: Hardcover

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Modern HPLC for Practicing Scientists

ASIN: 1856174662

Book Description

This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.

This book
Provides practical, hands-on advice based on years of experience
Will help ensure optimal design, equipment and separation results for your particular task
Presents system layouts from laboratory to process scale
Will help you to devise or improve record-keeping and documentation systems

·Provides practical, hands-on advice based on years of experience
·Will help ensure optimal design, equipment and separation results for your particular task
·Presents system layouts from laboratory to process scale
·Will help you to devise or improve record-keeping and documentation systems

HPLC Made to Measure: A Practical Handbook for Optimization

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HPLC Made to Measure: A Practical Handbook for Optimization

Manufacturer: Wiley-VCH
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ASIN: 352731377X

Book Description

The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness.
This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.

Practical High-Performance Liquid Chromatography

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Practical High-Performance Liquid Chromatography
Veronika R. Meyer
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Paperback

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ASIN: 0470093781

Book Description

Veronika Meyer’s book on HPLC is a classic text and remains one of the few titles available on general HPLC. Following on from the success of the previous three editions, this new, fourth edition continues to provide users of HPLC in industry, government, and service laboratories, as well as postgraduate students, with a unified approach to HPLC and an equal treatment of the theory and practice of this important technique.

The contents of this edition have been revised, expanded and updated. Where available, old literature references have been replaced by recent ones. New sections on the following topics have been included:

Shelf-live of mobile phases
The mixing cross
Phase systems in ion chromatography
Measurement uncertainty

Average customer rating:

It was a very informative book

Practical High-Performance Liquid Chromatography
Veronika R. Meyer
Manufacturer: John Wiley & Sons
ProductGroup: Book
Binding: Paperback

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Book Description

Practical High-Performance Liquid Chromatography Third Edition Veronika R. Meyer Swiss Federal Laboratories for Materials Testing and Research, St. Gallen, Switzerland Veronika Meyer's book is a classic HPLC text and remains one of the few titles on general HPLC. Following on from the excellent success of the first two editions, this third edition continues to provide postgraduate students using HPLC and users of HPLC in industry/pharmaceuticals with a unified approach to HPLC and an equal treatment of the theory and practice of HPLC. This edition provides numerous additions and updated material, including:
* 10 out of 26 chapters substantially revised
* Synopsis of the most important formulas
* lnclusion of chapters on solvent properties and on instrument tests
* Completely rewritten chapter on pumps
* Updated figures and references

Customer Reviews:

It was a very informative book.......1998-11-08

As I am studying analytical chemistry in Ireland, I found this book very helpful. It was well worth buying as it helped me a lot when studying for my exams.

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