Protein NMR Spectroscopy: Principles and Practice

Book Description

Protein NMR Spectroscopy combines a comprehensive theoretical treatment of NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules in solution. Beginning with simple theoretical models and experimental techniques, Protein NMR Spectroscopy develops the complete repertoire of theoretical principles and experimental techniques necessary for understanding and implementing the most sophisticated NMR experiments.

Important new techniques and applications of NMR spectroscopy have emerged since the first edition of this extremely successful book was published in 1996. The second edition includes new sections describing measurement and use of residual dipolar coupling constants for structure determination, TROSY and deuterium labeling for application to large macromolecules, and experimental techniques for characterizing conformational dynamics. In addition, the treatments of instrumentation and signal acquisition, field gradients, multidimensional spectroscopy, and structure calculation are updated and enhanced.

Protein NMR Spectroscopy is written as a graduate-level textbook and will be of interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy or who wish to understand the latest developments in this field.

· Provides an understanding of the theoretical principles important for biological NMR spectroscopy
· Demonstrates how to implement, optimize and troubleshoot modern multi-dimensional NMR experiments
· Allows for the capability of designing effective experimental protocols for investigations of protein structures and dynamics
· Includes a comprehensive set of example NMR spectra of ubiquitin provides a reference for validation of experimental methods

Customer Reviews:

Satisfied.......2007-09-05

I have been reading this book for a while and decided to buy it. I am satisfied with the vendor, it arrived in good condition and fairly on time.

New 'Bible' for Protein NMR.......2007-06-16

This is the new standard for Protein NMR spectroscopists (the old one being Ernst). Everyone I've met who uses NMR in biology has nothing but praise for this book. It's not a perfect book and has some weakness, especially in the modelling portions. The math derivations are clear but the authors do not connect back to the physical phenomenon. I would recommend keeping a QM book nearby as a reference. However, this book is well written overall and very complete. Highly recommended!

Useful Handbook for Protein NMR study.......2000-04-29

The book is very complete, covers most of the field in protein NMR studies. The book is well organised, with plenty of figures to facilitate easy understanding of the subject. Overall, I find it is very helpful for anyone who will be interested in protein NMR research. I have actually seen quite a number of copies in a few NMR laboratories and I would recommand this book to all those who are looking for an complete introductory book in NMR and protein study.

Bioreactors for Tissue Engineering: Principles, Design and Operation

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Bioreactors for Tissue Engineering: Principles, Design and Operation

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 1402037406

Book Description

Developments in tissue engineering for human medicine are increasing rapidly. Advances in stem cell biology, biomaterials science and scaffold design underpin this emerging science. An equally important facet of this field is the rational design and operation of bioreactors to control the nascent tissue growth. For the first time in a single volume, the design, characterisation and operation of the bioreactor system in which the tissue is grown is detailed. Bioreactors for Tissue Engineering presents an overall picture of the current state of knowledge in the engineering of bioreactors for several tissue types (bone, cartilage, vascular), addresses the issue of mechanical conditioning of the tissue, and describes the use of techniques such as MRI for monitoring tissue growth. This unique volume is dedicated to the fundamentals and application of bioreactor technology to tissue engineering products. Not only will it appeal to graduate students and experienced researchers in tissue engineering and regenerative medicine, but also to tissue engineers and culture technologists, academic and industrial chemical engineers, biochemical engineers and cell biologists who wish to understand the criteria used to design and develop novel systems for tissue growth in vitro.

Molecular Fluorescence: Principles and Applications

Average customer rating:

great for beginners, but too few references

Molecular Fluorescence: Principles and Applications
Bernard Valeur
Manufacturer: Wiley-VCH
ProductGroup: Book
Binding: Hardcover

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ASIN: 352729919X

Book Description

Today, fluorescence spectroscopy is an important tool of investigation in many areas. In analytical sciences, its advantage is extremely high sensitivity and selectivity - even single molecules can be detected - and it achieves a high spatial resolution and time resolution in combination with microscopic techniques or laser techniques, respectively. In material sciences, this is used to study structure and dynamics of surfaces. Particularly in the areas of biochemistry and molecular genetics, fluorescence spectroscopy has become a dominating technique. Together with the latest imaging techniques, fluorescence spectroscopy allows a real-time observation of the dynamics of intact biological systems with an unprecedented resolution.
This book offers a comprehensive introduction to and survey of fluorescence spectroscopy. It is written for newcomers and active researchers alike who are learning to apply fluorescence methods in the areas of chemistry, physical chemistry, polymers, materials, colloids, biochemistry, biology, medical and pharmaceutical research.

Customer Reviews:

great for beginners, but too few references.......2003-12-07

This is a great introductory book. Beautifully edited, very well written, with a lot of information to start digging each subject; I just missed a few more references in each chapter, and also more references to journals and not just other books, and then I would have been a 5 star.

Advanced Biological Treatment Processes for Industrial Wastewaters: Principles and Applications

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Advanced Biological Treatment Processes for Industrial Wastewaters: Principles and Applications

Manufacturer: IWA Publishing
ProductGroup: Book
Binding: Hardcover

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ASIN: 1843391147

Book Description

Advanced Biological Treatment Processes for Industrial Wastewaters provides unique information relative to both the principles and applications of biological wastewater treatment systems for industrial effluents. Case studies document the application of biological wastewater treatment systems in different industrial sectors such as chemical, petrochemical, food-processing, mining, textile and fermentation. With more than 70 tables, 100 figures, 200 equations and several illustrations, the book provides a broad and deep understanding of the main aspects to consider during the design and operation of industrial wastewater treatment plants. Students, researchers and practitioners dealing with the design and application of biological systems for industrial wastewater treatment will find this book invaluable.

Metabolic Engineering: Principles and Methodologies

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Excellent

Metabolic Engineering: Principles and Methodologies
Gregory N. Stephanopoulos , Aristos A. Aristidou , and Jens Nielsen
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0126662606

Book Description

Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes.
Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems.

Key Features
* Demonstrates metabolic engineering in action with numerous examples of pathway modification
* Includes methods for identifying key enzymes in metabolic networks
* Contains a comprehensive review of metabolic biochemistry
* Discusses metabolic regulation at the gene, enzyme, operon, and cell levels
* Explains concepts of stoichiometry, kinetics, and thermodynamics of metabolic pathways
* Minimizes mathematical complexity
* Links to a Web page to communicate updates of the software code and homework problems

Customer Reviews:

Excellent.......2006-08-21

This book was a very pleasant surprise. It is rigurous yet accessible enough to provide a very clear picture of the metabolic pathway engineering field. Complicated topics like metabolic control and flux analisys (MCA) are very well treated. The examples are usually relevant and well explained, however, some need pen and paper to work up some jumps in the reasoning train. The first chapter is just an introduction whereas the second chapter is a review of cellular metabolism that may result too dense for most people (including myself). Most biologists can probably do away skipping it (althought some examples involving transport where quite interesting). I am glad I didn't put the book away because on chapter 3 things get really exciting. If you are interested in understanding how metabolic pathways are organized and what analytical tools are there available to model them (and eventually "reconfigure") this book for you.

Note: the book is meatn to be undestood and it is nice to see MCA depleted of the hoopla many authors insist on surrounding it with.

Average customer rating:

Excellent
The Luminescence Bible
A most worthy successor to the first edition
Great book, new edition out soon
An excellent book for an overview on fluorometry

Principles of Fluorescence Spectroscopy
Joseph R. Lakowicz
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Accessories:

ASIN: 0306460939

Book Description

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Customer Reviews:

Excellent.......2007-05-22

Stellar text. Very thorough and easy to read. New version is in color, and has several new chapters. I'm very happy with the new edition

The Luminescence Bible.......2007-03-09

What's more to say? This is the authoritative, the definitive volume for luminescence experiments from theory to all of the most useful applications. It is readable, dense, useful and indispensable if you do fluorescence, lifetime measurements, energy transfer studies, etc., etc.

A most worthy successor to the first edition.......1999-09-14

In the sixteen years since the appearance of the first edition of Lakowicz's Principles of Fluorescence Spectroscopy, the use of fluorescence as an analytical tool has grown remarkably, especially in instrumentation, available fluorophores, and practical applications. Therefore, it shouldn't be surprising that the second edition of the work has more than doubled in size.

The approach is the same as that in the first edition: fluorescence spectroscopy from the viewpoint of a biophysical chemist. Explanations are clear. References are profuse; they now number well over a thousand and include an additional appendix that cites many books and review articles for additional reading. Figures are also plentiful; no fancy color diagrams a la contemporary biochemistry texts, but basic line drawings, primarily adapted from journal articles.

The first three chapters ("Introduction to Fluorescence", "Instrumentation for Fluorescence Spectroscopy", and "Fluorophores") are an excellent brief (93-page) introduction to the whole field. Most of the remaining 19 chapters are organized around the kinds of photophysical processes and spectroscopic quantities that are relevant to fluorescence. These include very thorough treatments of fluorescence lifetimes, quenching, anisotropy, and energy transfer. There are many examples drawn from journal articles. In addition, the work now covers more of the technological applications of fluorescence, especially in chapters on "Fluorescence Sensing" and "DNA Technology". These give a sense of the great contributions that fluorescence has made to biotechnology.

My own work involves varied applications of fluorescence to biotechnology, and the second edition of Lakowicz is my reference book of choice. It would also be an excellent text for a graduate course on fluorescence in a chemistry or biochemistry department. All in all, this is a most worth successor to the first edition.

Great book, new edition out soon.......1999-06-10

Joe Lakowicz has just finished a thorough revision of this classic work, which should be coming out soon. Highly recommended.

An excellent book for an overview on fluorometry.......1999-05-12

Introduces the reader to the basic physics and physical chemistry of fluorescence phenomena, fluorometry techniques and covers advanced topics as well. An excellent textbook.

Physical Chemistry of Macromolecules: Basic Principles and Issues

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Physical Chemistry of Macromolecules: Basic Principles and Issues
S. F. Sun
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Hardcover

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ASIN: 0471281387

Book Description

This book includes end of chapter problems and references, and also:

Enables users to improve basic knowledge of biophysical chemistry and physical polymer chemistry.

Explores fully the principles of macromolecular chemistry, methods for determining molecular weight and configuration of molecules, the structure of macromolecules, and their separations.

Download Description

Integrating coverage of polymers and biological macromolecules into a single text, Physical Chemistry of Macromolecules is carefully structured to provide a clear and consistent resource for beginners and professionals alike. The basic knowledge of both biophysical and physical polymer chemistry is covered, along with important terms, basic structural properties and relationships.
This book includes end of chapter problems and references, and also:
Enables users to improve basic knowledge of biophysical chemistry and physical polymer chemistry.
Explores fully the principles of macromolecular chemistry, methods for determining molecular weight and configuration of molecules, the structure of macromolecules, and their separations.

Biomechanics: Principles and Applications

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Biomechanics: Principles and Applications

Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 0849314925

Book Description

The ultimate objective of biomechanics is to define the normal envelope of anatomic and physiologic responses to distributed systems of external and internal forces, for both medical and nonmedical applications. Biomechanics: Principles and Applications offers a definitive, comprehensive review of all the major disciplines in this rapidly growing field, including recent advancements made by biomedical engineers to the understanding of fundamental aspects of physiologic function in health, disease, and environmental extremes. The chapters, each discussed by a recognized world leader in the respective field, address the subjects of biosolid mechanics and biofluid mechanics as they pertain to various subsystems of the human body. A unique feature is the convenient handbook style and tabular format that puts quantitative data at your fingertips. Illustrations further add to the value of this book. The text is concise, topical, and not overly technical. No other book covers the entire field of biomechanics so succinctly in one volume.

Principles and Processes for Evaluating Endocrine Disruption in Wildlife (Setac Technical Publications Series)

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Principles and Processes for Evaluating Endocrine Disruption in Wildlife (Setac Technical Publications Series)
Chemical Manufacturers Association (U. S.) , and SETAC (Society)
Manufacturer: Setac Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1880611171

Book Description

Endocrine disruption in wildlife has emerged as a significant factor in scientific debate about environmental policy. Using a risk-based approach, Principles and Processes for Evaluating Endocrine Disruption in Wildlife provides state-of-the-art information, principles, and processes for evaluating the effects of endocrine-disrupting chemicals in wildlife. Although the topic of environmental endocrine disruption is one that has generated significant controversy, it is evident from available scientific data that endocrine disruption, including developmental anomalies and disruption in reproductive processes, may be of concern in birds, mammals, and other wildlife, including fishes. Principles and Processes for Evaluating Endocrine Disruption in Wildlife is a valuable reference tool for educational purposes as well as for environmental and industrial managers and planners, regulators, environmental and human health scientists, public health workers, and people involved in environmental law and policy. This timely document resulted from a major international workshop made up of scientists from academia, government, and industry, and it is a significant contribution to decision-making in both environmental science and policy considerations involving endocrine-disrupting chemicals and wildlife.

Principles and Practices of in Situ Chemical Oxidation Using Permanganate

Average customer rating:

Excellent & clear
Excellent

Principles and Practices of in Situ Chemical Oxidation Using Permanganate

Manufacturer: Battelle Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1574771027

Customer Reviews:

Excellent & clear.......2003-02-20

It is very clear that Principles of In-Situ Chemical Oxidation

Excellent.......2002-10-08

This book provides a very clear and comprehensive approach to in situ permanganate oxidation. The book was extremely useful and I would highly recommend.

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