Customer Reviews:
Epigenetics - a timely and important text........2007-05-15
It is becoming clear that epigenetic mechanisms are responsible for a large number of "genetic" events in the organism, both under normal and pathological circumstances. This advanced text (suitable for advanced undergraduates and beginning graduate students) brings together, in a very readable way, the various mechanisms of epigenetic heredity.
An invaluable educational tool.......2007-04-11
Epigenetics is a scientific textbook written to teach advanced undergraduate and graduate college students about epigenetic mechanisms, which regulate gene expression in many biological processes. Chapters cover histone modifications, chromatin remodeling, DNA methylation, siRNAs and gene silencing, X-chromosome inactivation, epigenetics in microbes, plants, insects and mammals, and much more. An in-depth, scholarly, and informative instructional reference, interspersed with occasional color photographs and diagrams, featuring an extensive list of references and a general summary at the end of each chapter to aid comprehension. An invaluable educational tool for specialized college science courses, as well as for professionals in the field of biology seeking to sharpen their understanding of modern epigenetics.
Represents the Current State of the Art.......2006-12-19
Wikipedia says: 'Epigenetics is the study of epigenetic inheritance, a set of reversible heritable changes in gene function or other cell phenotype that occur without a change in DNA sequence (genotype).'
That's a pretty general definition, and you can get into some pretty good arguments that this or that word should be changed.
The fundamental discoveries that began the field began to be discussed in 1941, but it grew slowly until fairly recent years. By 2004 the science had developed enough that the 69th Cold Spring Harbor Symposium on Quantitative Biology covered the topic. Many of the authors of this book attended that symposium and the seeds for this book were sown.
The book was written by about forty of the preeminent researchers in the field. It consists of 24 chapters covering virtually all known aspects of the subject. It can serve as a reference work for the current state of the art, or could be used as a text for advanced undergraduate or graduate courses on gene regulation.
Average customer rating:
- A good introduction to the topic
- Well written, short explanations but nevertheless understandable
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Microarray Gene Expression Data Analysis: A Beginner's Guide
Helen Causton ,
John Quackenbush , and
Alvis Brazma
Manufacturer: Blackwell Publishing Limited
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Data Analysis Tools for DNA Microarrays
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Bioinformatics For Dummies (For Dummies (Math & Science))
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Guide to Analysis of DNA Microarray Data, Second Edition
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The Analysis of Gene Expression Data
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Statistical Analysis of Gene Expression Microarray Data
ASIN: 1405106824 |
Book Description
Microarray technology is arguably the most important recent breakthrough in molecular biology. It enables researchers to obtain snapshots of gene expression for all the genes in a genome in a single experiment. Microarray experiments generate massive amounts of data that can be analysed to extract new knowledge about the underlying biological processes.This guide covers aspects of designing microarray experiments and analysing the data generated, and includes information on some of the tools that are available from non-commercial sources. Concepts and principles underpinning gene expression analysis are emphasised, and wherever possible the mathematics has been simplified. The guide is intended for use by graduates and researchers in bioinformatics and the life sciences and is also suitable for statisticians who are interested in the approaches currently used to study gene expression.
Customer Reviews:
A good introduction to the topic .......2007-05-20
Microarrays are a tool for monitoring gene expression levels for thousands of genes in parallel. This technology is very useful since patterns in the gene expression can be used for molecular characterization of phenomena that range from disease states and response to stimuli to the differences between cells of different types. The amount of information obtained from one microarray experiment can be large. These large amounts of information present new challenges in the areas of data storage, management, and analysis by biologists who are not accustomed to dealing with this much data. Also, the software used for data analysis is usually written by mathematicians and statisticians that have a minimum of training in biology.
This book addresses some of the issues faced by researchers who are beginning their first microarray experiments. It covers various aspects of designing and analyzing the results of microarray experiments. Microarrays are not limited to the study of gene expression, but this remains the most common use of the technology and therefore is the only use of arrays discussed here. This book attempts to explain the underlying concepts and principles routinely used in analysis of gene expression data. The book should be accessible by statisticians, computer scientists, and students of bioinformatics who want a grounding in the types of analysis currently used to study microarray data.
The book begins with an introductory chapter which is followed by three major chapters. As with any technology that has the capacity to detect small changes in a highly dynamic system, the underlying experimental design and the manner in which an experiment is conducted is critical for obtaining high quality data. Chapter two addresses these issues. The raw data from microarray experiments are images that must be transformed and organized into gene expression matrices. These transformations are the subject of chapter 3. Finally, in chapter 4, the common methods used for analyzing gene expression data matrices with the goal of obtaining new insights into biology are discussed. The book does a pretty good job of providing the reader with a general understanding of the nature of microarray data and how it can be analyzed. It was never meant to be a reference book or a comprehensive review, just a gentle introduction.
Well written, short explanations but nevertheless understandable.......2005-07-06
Certainly, this book can not give a complete description of microarrays, neither from an experimental nor a theoretical side. Nevertheless, the issues presented and discussed provide the reader with a solid basis for more advanced studies.
In my opinion, this book is well written, the explanations given are descriptive and understandable and its overall organization is plausible. I recommend this book as an introduction for the analysis of microarray data, because it provides a good overview of existing methods in this field. A warning: This does not mean, that all these methods are thorougly expained! It just provides an overview!! If you want to learn, e.g., clustering methods, you should consult another book (probably no other book about microarrays but a decent book dealing only with data analysis in general or clustering methods...)
Book Description
By the best selling author of The Selfish Gene 'This entertaining and thought-provoking book is an excellent illustration of why the study of evolution is in such an exciting ferment these days.' Science 'The Extended Phenotype is a sequel to The Selfish Gene . . . he writes so clearly it could be understood by anyone prepared to make the effort' John Maynard Smith, London Review of Books 'Dawkins is quite incapable of being boring this characteristically brilliant and stimulating book is original and provocative throughout, and immensely enjoyable.' G. A. Parker, Heredity 'The extended phenotype is certainly a big idea and it is pressed hard in dramatic language.' Sydney Brenner, Nature 'Richard Dawkins, our most radical Darwinian thinker, is also our best science writer.' Douglas Adams 'Dawkins is a superb communicator. His books are some of the best books ever written on science.' Megan Tressider, Guardian 'Dawkins is a genius of science popularization.' Mark Ridley, The Times
Customer Reviews:
Good info.......2007-08-23
Beware that this book is a lot more technical than the Selfish Gene, although Dawkins writes it in a similar fashion and includes a glossary for the tricky terms.
I did find it more repetitive than I was expecting as Dawkins really strives to drive the point home, but as a whole it's still a great book.
More delving for truth, less bashing falsehood.......2007-05-18
Lots of people seem to know about Dawkins as a Enthusiastic Bright, or at least as a 'staunch atheist'. Selfish Gene takes digs at religion. Blind Watchmaker certainly takes digs at religion. Ancestor's Tale even seems to have some (though I've not got all through it yet.) I haven't even opened "The God Delusion" yet, and I can make a good guess about the digging I'll find there.
The Extended Phenotype, on the other hand, is pretty well free of anti-religious side commentary. It reads much more like a scientist making a case to his peers, and less like a science writer trying to explain things to a nonscientific audience. That means freedom from much of any religious commentary (I don't remember any, so if there was some, it was minor), but it also means the writing style is a little less conversational. It still feels recognizably Dawkins to me, but the emphasis is more on making his point and less on getting people to listen.
I'd say it's a less /fun/ read than, for example, Blind Watchmaker or Selfish Gene, but at the same time it's a more /interesting/ read.
Very extended.......2007-01-18
Thought book was very well written, although it was hard to follow in some places. But this is a more detailed account of evolution and how the gene and DNA make up what we our today. Overall great work done by one of the best scientists in the field today.
Injustamente esquecido.......2007-01-10
Este é um dos livros de referência do Richard Dawkins mas que é frequentemente relegado como uma obra secundária. Este livro traz uma ótica nova sobre a relação entre genótipo e o fenótipo e em última análise com o indivíduo, redefinindo praticamente este último conceito.
Para os leitores tardios do autor, este livro pode parecer muito técnico já que foi escrito após O Gene Egoísta e visava reforçar alguns pontos de vista desta obra e introduzir todo um novo conceito de fenótipo, isto torna a leitura mais acessível para os que têm maior fluência nas ciências biomédicas mas em momento algum compromete o entendimento da questão central do livro pelo público interessado em ciência mas mais leigo nesta área.
Difficult but eminently worthwhile.......2006-12-30
This is a long and difficult book, although not as long and difficult as it might be if it had been written by somebody without Richard Dawkins' gift for clarity of thought and expression.
The crux of Dawkins' thesis is expressed early on and much of what follows is a very detailed supporting argument. What he wants us to see is that the "selfish gene" has a reach that extends beyond the confines of the individual organism that houses the gene. The phenotype of our genes is the human organism in all its glory; however the extended phenotype of our genes is not only the human organism but part of the environment in which the organism finds itself. In other words, the gene has the power to influence not only our behavior but the behavior and structure of elements in the world in which we live.
This thesis is not as striking to me as it has been to many others mainly because I have studied Eastern religious views, and it is a tenant of such views that the distinction between ourselves (the "selfish organism," in Dawkins' terminology) and the environment is an artificial one, an illusion actually. We are part and parcel of all that is around us and within us, and the boundary of our skin is merely functional. We cannot be understood by looking at only our bodies. Dawkins makes the point that looking at a beaver and microscopically examining it and its genes is not sufficient to an understanding of what a beaver is. We have to also consider the dams that the beaver builds, the trees that it gnaws down and even the streams that it dams and turns into lakes.
Presenting a point of view somewhat at odds with that of Dawkins (and one that I think that Dawkins does not sufficiently appreciate) is Franklin M. Harold in his book, The Way of the Cell: Molecules, Organisms and the Order of Life (2001). He writes, "Organisms process matter and energy as well as information; each represents a dynamic node in a whirlpool of several currents, and self-reproduction is a property of the collective, not of genes.... DNA is a peculiar sort of software, that can only be correctly interpreted by its own unique hardware.... [S]ending aliens the genome of a cat is no substitute for sending the cat itself--complete with mice." (p. 221)
Dawkins tries to discount the view of those he calls "group selectionists" who see life from a "group benefit" viewpoint. Dawkins has, since writing this book, stepped back from this position to allow that some group selection may take place. I believe some day he may see the world not from a "selfish gene" point of view, and not from a "selfish organism" point of view, but from a "selfish ecosystem" perspective--well, more likely his successors will see this, since the work of a lifetime is not easily amended in one's later years.
Dawkins gives what he calls "our own 'central theorem' of the extended phenotype" on page 233: "An animal's behaviour tends to maximize the survival of the genes 'for' that behaviour, whether or not those genes happen to be in the body of the particular animal performing it."
This is a mouthful. Clearly we can say that the genes of the reed warbler code for behavior that benefits the genes of the cuckoo who has laid its egg in the warbler's nest. This is what Dawkins has in mind. But then arises the question, "how far afield can the phenotype extend?" Here Dawkins gets cautious and writes, "The farthest action at a distance I can think of is a matter of several miles." (p. 233) Note the chosen terminology, "action at a distance." This is from physics of course causing Dawkins to ask if there is "a sharp cut-off" of the genes' reach or "an inverse square law" at work?
It is here that I believe Dawkins has come so, so close to that which he will not see (or couldn't see then), namely that everything works toward an ecology and that the idea of selfish genes and selfish organisms is a limited view. In truth the reach of the genes should be governed by something like an inverse square law since humans are now reaching beyond the solar system.
When we look at such great distances we might want to credit the dreaded and verboten "group selection" that Dawkins is at pains to reject. Just as some see our earth as "Gaia," an organism itself, so too might we see those organisms that have the means to survive the destruction of the home planet by migrating to other planets as being selected by group as opposed to other groups who have no such ability. Planet A produces beings that extend beyond their solar system; planet B produces beings that do not. Both planets blow up. Who is "selected" by the (extended) environment and who is not?
Dawkins is one of the geniuses of science, and I don't mean to argue with the great insights he has brought to biology, but my point is that it is always something of an artificiality to speak of living systems as confined to one level of existence or expression. We may think of earth creatures as being completely separate from the rest of the universe, yet without the sun, 93 million miles away, we would not exist; and come a supernova even many light years away, we will be affected.
So all is one and one is all in some extended sense. And using the word "selfish" (as Dawkins knows) at any level of life is merely to be anthropomorphic.
Daniel Dennett, in a new afterword written in 1999, asks if this book is science or philosophy, and he answers both. I agree, and it is science and philosophy of the highest order, aimed equally at the professional and at the educated layperson.
Book Description
Outlines the relationship between genes and human experience. The understandings of gene expression emerging from the Human Genome Project are setting the stage for a profound expansion of our understanding of life. We are just now beginning to learn how the brain, body, and genes interact in everyday life. Here, Ernest Rossi introduces the new science of psychosocial genomics and explores how it will profoundly change our understanding of the pathways of communication among mind, body, and spirit.
Customer Reviews:
A Sourcebook for Therapy in the 21st Century.......2004-01-29
Dr. Rossi has again written a "trail blazing" book. Those familiar with his previous publications know the breadth of his work - a work spanning Ericksonian Hypnosis, Jungian Psychology, Mind-Body Healing, Biological rhythms, and the psychology of Dreaming. He has not written a book on mathematical models of healing but it is clear that he has also studied this area in considerable depth. All the subjects listed above are revisited and taken to new levels in this book.
In addition to these sections Dr. Rossi also shares some very clear and vivid descriptions of actual therapuetic work. In one section he gives an entire transcription of a therapy he did with a woman during a workshop. In another section he lists a series of therapeutic exercises that can be used to access each person's deeper capacities for self healing.
This latest book also places Dr. Rossi in the ranks of the great detectives. He has been systematically tracking down all of the current research relating experience to gene expression. He regularly scans the internet for all sources that may have new findings related to this subject. He asks us to remember that this is "work in progress." Investigators all over the world are unlocking how specific genes are "turned on" by different experiences. Dr. Rossi is the only person I know who is making an attempt to synthesize this information within in a larger psychological context.
Currently both our medical culture and our larger culture continues to propagate extremely primitive models of human development. According to one of the most popular we inherit certain "tendencies" or diseases that are expressed in a nervous system that is essentially fixed after birth. Thus, our best therapeutic efforts will be directed towards modifying the brains that have genetically predestined defects. With these assumptions pharmacology will be the main (often only) ingredient of help.
At the beginning of the book Dr Rossi points out that recent findings have overturned many of our old theories about how the nervous system is linked to experience. We now know that novelty, life-enriching experiences and physical exercise can activate neurogenesis. Such experiences can turn on gene expression within minutes throughout the body and brain. As a result, "every memory is a reframe." We can actually reconstruct ourselves from a genetic level on up when we are experiencing a healing environment.
Taken as a whole Dr. Rossi has written one of the great sourcebooks for healing to guide us into the 21st Century.
Peak of a brilliant career?.......2003-09-10
I mean this review to be brief so I'll state my conclusion at the start: this is an excellent book covering biochemistry up to psychotherapy. It is a must for any Rossi fan, if only because it has that 'culmination-of-a-brilliant-career' feel to it.
I discovered Rossi in the early 1980's and took one of his "64 questions in search of a graduate student" as my master's thesis. Since then I have digested his work and watched, sometimes in amazement, as clients changed and as I changed. Rossi's view of the mind-body in therapy works and is workable.
I had the pleasure of seeing him in Texas this summer (2003) and was mesmerized (pardon the pun) by the obvious skill of a master therapist. Between his demo to our group and reading this book with a view to integrate it into my work, I have found a revitalizing of my therapy practice that has pulled me out of a 2-year slump... enough said.
Thanks Dr. Rossi.
Excellent integration of biology and mind.......2003-05-01
As a clinical psychologist with a background in biochemistry, I am very thrilled to see the author to integrate the gene expression, environment and mind into a coherent and scientifically-based picture. The book offers a solid foundation for the biological bases for psychotherapy and provides a holistic viewpoint upon human activities. It is highly recommended !
Customer Reviews:
The State of the Art as of Late 2006.......2007-03-07
This book describes a series of protocols that offer ways to deliver genes into mammalian cells. About half the book describes viral techniques where a virus is used to do the transport and delivery. The other half describes non-viral methods. The book has some 75 different techniques, each described by a researcher who has been using/developing that method.
Note that many of these techniques are brand new and still in the stage of being developed. Some of them are likely to prove useful, others will be of limited use, perhaps in a niche application or will simply lead to better techniques later on.
Dr. Rossi (Bechmann Research Institute) and Dr. Friedmann (UCSD) serve as editors of the book. It is called a Laboratory Manual as the protocols are presented in a stepwise, 'cookbook' style.
The book was published in early 2007 and should be considered an accurate reflection of the state of the art as of late in 2006.
Average customer rating:
- An excellent source of practical information
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Baculovirus Expression Vectors: A Laboratory Manual
David R. O'Reilly ,
Lois K. Miller , and
Verne A. Luckow
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Baculovirus and Insect Cell Expression Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)
ASIN: 0195091310 |
Book Description
Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriate baculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelines for choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensive approach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. The manual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
Customer Reviews:
An excellent source of practical information.......1998-04-03
This book is a must-have laboratory reference for researchers who want to use insect cell expression systems in their labs. It includes an introduction to baculovirus biology and insect cell culture, descriptions of baculovirus vectors, and laboratory protocols.
Book Description
The interdisciplinary nature of bioinformatics presents a challenge in integrating concepts, methods, software, and multi-platform data. Although there have been rapid developments in new technology and an inundation of statistical methodology and software for the analysis of microarray gene expression arrays, there exist few rigorous statistical methods for addressing other types of high-throughput data, such as proteomic profiles that arise from mass spectrometry experiments. This book discusses the development and application of Bayesian methods in the analysis of high-throughput bioinformatics data, from medical research and molecular and structural biology. The Bayesian approach has the advantage that evidence can be easily and flexibly incorporated into statistical models. A basic overview of the biological and technical principles behind multi-platform high-throughput experimentation is followed by expert reviews of Bayesian methodology, tools, and software for single group inference, group comparisons, classification and clustering, motif discovery and regulatory networks, and Bayesian networks and gene interactions.
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Methods in Enzymology, Volume 306: Expression of Recombinant Genes in Eukaryotic Systems (Methods in Enzymology)
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ASIN: 0121822079 |
Book Description
The critically acclaimed laboratory standard for more than forty years,
Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Analysis of Gene Expression
* Gene Expression Systems for Functional Analysis
* Yeast Expression Systems
* RNA-based Control of Recombinant Gene Expression
* Small Molecule Control of Gene Expression
* Viral Systems for Recombinant Gene Expression
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A Beginner's Guide to Microarrays
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Computer Viruses and Malware (Advances in Information Security)
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The Physics of Quantum Information: Quantum Cryptography, Quantum Teleportation, Quantum Computation
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Introduction to Cryptography: Principles and Applications (Information Security and Cryptography)
ASIN: 1402074727 |
Book Description
Microarray technology is more accessible than ever, and an ever-widening field of scientists is using this technology. However, the manufacture, experimental design, and analysis of microarrays are not always straightforward, and researchers new to the field run into technical and theoretical roadblocks that can hinder progress with this powerful new technology.
A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:
-Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols.
This book is addressed to researchers using microarrays for the first time. One faces a myriad of problems at the outset of such a task, and there is no need to 'reinvent the wheel' for each scientist that runs into these problems. Knowing the strengths and weaknesses of microarrays before research begins can save time, money, and resources.
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Hox Gene Expression (Molecular Biology Intelligence Unit)
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ASIN: 0387689893 |
Book Description
The study of Hox genes is crucial not only in exploring the enigma of homeosis but also in understanding normal development at the fundamental molecular level. "Hox Gene Expression" starts with the amazing discovery of the homeobox twenty-three years ago and follows the exciting path thereafter of a series of breakthroughs in Genetics, Development and Evolution. It deals with homeotic genes- their evolution, structure, normal and abnormal function. Researchers and graduate students in Biology and Medicine will benefit from this integrated overview of Hox gene activities.
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Books Index
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